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Recombinant UL30 antigen-based single serum dilution ELISA for detection of duck viral enteritis.

Abstract：

:A recombinant UL30 antigen-based single serum dilution enzyme linked immunosorbent assay (ELISA) was developed to measure specific antibody in the sera of ducks against duck enteritis virus (DEV). The partial UL30 gene of DEV was cloned, expressed, purified and tested for its diagnostic use by designing a single serum dilution enzyme linked immuno-sorbent assay (ELISA). A total of 226 duck sera samples were tested using the assay. A linear relationship was found between the predicted antibody titres at a single working dilution of 1:100 and the corresponding serum titres observed as determined by the standard serial dilution method. Regression analysis was used to determine a standard curve from which an equation was derived which demonstrated this correlation. The equation was then used to convert the corrected absorbance readings of the single working dilution directly into the predicted ELISA antibody titres. The assay proved to be specific, sensitive and accurate as compared to the virus neutralization test with a specificity, sensitivity and accuracy being 96%, 95% and 95% respectively.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Aravind S,Patil BR,Dey S,Mohan CM

doi

10.1016/j.jviromet.2012.06.027

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

234-8

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(12)00240-6

journal_volume

185

pub_type

杂志文章

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