Development of a novel rapid immunochromatographic test specific for the H5 influenza virus.


:Three anti-H5 influenza virus monoclonal antibody (mAb) clones, IFH5-26, IFH5-115 and IFH5-136, were obtained by immunising a BALB/C mouse with inactivated A/duck/Hokkaido/Vac-1/04 (H5N1). These mAbs were found to recognise specifically the haemagglutinin (HA) epitope of the influenza H5 subtypes by western blotting with recombinant HAs; however, these mAbs have no neutralising activity for A/duck/Hokkaido/84/02 (H5N3) or A/Puerto Ric/8/34 (H1N1). Each epitope of these mAbs was a conformational epitope that was formed from the regions located between 46 to 60 amino acids (aa) and 312 to 322 aa for IFH5-115, from 101 to 113 aa and 268 to 273 aa for IFH5-136 and from 61 to 80 aa and 290 to 300 aa for IFH5-26. The epitopes were located in the loop regions between the receptor region and alpha-helix structure in haemagglutinin 1 (HA1). Influenza A virus H5-specific rapid immunochromatographic test kits were tested as solid phase antibody/alkaline phosphate-conjugated mAb in the following three combinations: IFH5-26/IFH5-115, IFH5-136/IFH5-26 and IFH5-136/IFH5-115. In every combination, only influenza A H5 subtypes were detected. For effective clinical application, rapid dual discrimination immunochromatographic test kits in combination with H5 HA-specific mAb, IFA5-26 and IFA5-115 and the influenza A NP NP-specific mAb, FVA2-11, were developed. The dual discrimination immunochromatographic tests kits detected influenza A virus H5 subtypes as H5 line-positive and all influenza A subtypes as A line-positive simultaneously. The dual discrimination immunochromatographic test kits may be useful for discriminating highly pathogenic avian influenza A H5N1 viruses from seasonal influenza A virus, as well as for confirming influenza infection status in human, avian and mammalian hosts.


J Virol Methods


Miyagawa E,Kogaki H,Uchida Y,Fujii N,Shirakawa T,Sakoda Y,Kida H




Has Abstract


2011-05-01 00:00:00














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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Okazaki K,Chiya S,Ideguchi S,Kimura S

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    abstract::A multiplex polymerase chain reaction (multiplex-PCR) to detect and differentiate bovine herpesvirus 1 (BoHV-1) and 5 (BoHV-5) was developed using primers for the gene sequence that encodes the glycoprotein C. The technique was assessed against the BoHV-1 and BoHV-5 cell culture adapted strains, and clinical samples c...

    journal_title:Journal of virological methods

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    authors: Claus MP,Alfieri AF,Folgueras-Flatschart AV,Wosiacki SR,Médici KC,Alfieri AA

    更新日期:2005-09-01 00:00:00

  • A novel HIV-1 reporter virus with a membrane-bound Gaussia princeps luciferase.

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    journal_title:Journal of virological methods

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    authors: Suree N,Koizumi N,Sahakyan A,Shimizu S,An DS

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    abstract::At 5 degrees C in phthalate buffer at pH 4.5, poliovirus 1 was destroyed 60 to 70% more rapidly by free chlorine (FC) in the presence of 1,262 mg/L (approximately 0.0169 M) KCl than in the absence of KCl. At 5 degrees C, more than 99.9% of FC exists as HOCl at pH 4.5. Since buffers themselves may have a marked potenti...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Berg G,Sanjaghsaz H

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hardy CT,Damrow TA,Villareal DB,Kenny GE

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Jeansson S,Elwing H,Nygren H,Olofsson S

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    authors: Park JS,Um J,Choi YK,Lee YS,Ju YR,Kim SY

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    abstract::The filter in situ hybridisation (FISH) method for detection of HPV in cervical swabs was evaluated against the Southern blot technique on concomitant cervical biopsies. Of 73 biopsies, HPV 16 DNA sequences were found in 26 biopsies and HPV 18 sequences in 2 biopsies. Analysis by FISH of the corresponding smears detec...

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    authors: Hørding U,Sebbelov A,Daugaard S,Bock JE,Norrild B

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  • Comparison of the Abbott RealTime High Risk HPV test and INNO-LiPA HPV Genotyping Extra test for the detection of human papillomaviruses in formalin-fixed, paraffin-embedded cervical cancer specimens.

    abstract::Comparative evaluation of Abbott RealTime and Innogenetics INNO-LiPA on alternately processed formalin-fixed, paraffin-embedded specimens of 31 cervical cancers and 31 uterine myomas showed complete agreement in the detection of 14 assay-common HPV genotypes and partial genotyping of HPV-16 and HPV-18. The tissue prep...

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    authors: Kocjan BJ,Seme K,Poljak M

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    abstract::Peste des petits ruminants virus (PPRV) causes a devastating disease of small ruminants present across much of Africa and Asia. Recent surveillance activities and phylogenetic analyses have suggested that the virus is an emerging problem as it is now being detected in areas previously free of the disease. As such, the...

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    authors: Batten CA,Banyard AC,King DP,Henstock MR,Edwards L,Sanders A,Buczkowski H,Oura CC,Barrett T

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    authors: Gaertner DJ,Winograd DF,Compton SR,Paturzo FX,Smith AL

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    authors: Schwarz BA,Bange R,Vahlenkamp TW,Johne R,Müller H

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    authors: Myc A,Anderson MJ,Baker JR Jr

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    authors: Xiang DX,Chen Q,Pang L,Zheng CL

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    authors: Shiraishi R,Hirayama N

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    authors: Honda ER,Zanchi F,Rios K,Lira E,DeusileneVieira,da Silva LH,De Paula SO

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    authors: Wong S,Pabbaraju K,Wong A,Fonseca K,Drews SJ

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    authors: Ben Salah A,Zaâtour A,Pomery L,Cohen BJ,Brown DW,Andrews N

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    authors: Maksyutov RA,Gavrilova EV,Meyer H,Shchelkunov SN

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    pub_type: 杂志文章


    authors: Pasquier C,Sandres K,Salama G,Puel J,Izopet J

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    authors: Boobalan J,Torti A,Dinesha TR,Solomon SS,Balakrishnan P,Saravanan S

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lee YJ,Park CK,Nam E,Kim SH,Lee OS,Lee du S,Lee C

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Mu B,Huang X,Bu P,Zhuang J,Cheng Z,Feng J,Yang D,Dong C,Zhang J,Yan X

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Grajkowska LT,Pedras-Vasconcelos JA,Sauder C,Verthelyi D,Puig M

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Steensels D,Reynders M,Descheemaeker P,Curran MD,Jacobs F,Denis O,Delforge ML,Montesinos I

    更新日期:2017-07-01 00:00:00

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Shiell BJ,Beddome G,Michalski WP

    更新日期:2002-04-01 00:00:00