Development of a novel rapid immunochromatographic test specific for the H5 influenza virus.


:Three anti-H5 influenza virus monoclonal antibody (mAb) clones, IFH5-26, IFH5-115 and IFH5-136, were obtained by immunising a BALB/C mouse with inactivated A/duck/Hokkaido/Vac-1/04 (H5N1). These mAbs were found to recognise specifically the haemagglutinin (HA) epitope of the influenza H5 subtypes by western blotting with recombinant HAs; however, these mAbs have no neutralising activity for A/duck/Hokkaido/84/02 (H5N3) or A/Puerto Ric/8/34 (H1N1). Each epitope of these mAbs was a conformational epitope that was formed from the regions located between 46 to 60 amino acids (aa) and 312 to 322 aa for IFH5-115, from 101 to 113 aa and 268 to 273 aa for IFH5-136 and from 61 to 80 aa and 290 to 300 aa for IFH5-26. The epitopes were located in the loop regions between the receptor region and alpha-helix structure in haemagglutinin 1 (HA1). Influenza A virus H5-specific rapid immunochromatographic test kits were tested as solid phase antibody/alkaline phosphate-conjugated mAb in the following three combinations: IFH5-26/IFH5-115, IFH5-136/IFH5-26 and IFH5-136/IFH5-115. In every combination, only influenza A H5 subtypes were detected. For effective clinical application, rapid dual discrimination immunochromatographic test kits in combination with H5 HA-specific mAb, IFA5-26 and IFA5-115 and the influenza A NP NP-specific mAb, FVA2-11, were developed. The dual discrimination immunochromatographic tests kits detected influenza A virus H5 subtypes as H5 line-positive and all influenza A subtypes as A line-positive simultaneously. The dual discrimination immunochromatographic test kits may be useful for discriminating highly pathogenic avian influenza A H5N1 viruses from seasonal influenza A virus, as well as for confirming influenza infection status in human, avian and mammalian hosts.


J Virol Methods


Miyagawa E,Kogaki H,Uchida Y,Fujii N,Shirakawa T,Sakoda Y,Kida H




Has Abstract


2011-05-01 00:00:00














  • An ELISA-inhibition assay for antibody to human immunodeficiency virus core antigen (p24).

    abstract::An ELISA-inhibition assay based on commercially available HIV-1 p24 antigen tests was developed for detecting p24 antibodies. The test is specific and simple. p24 antibody was detectable in all p24 antigen negative Western blot positive sera, but was detectable infrequently in antigen positive sera. Sera from patients...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Povolotsky J,Gold JW,Darminin P,Chein N,Baron P,Armstrong D

    更新日期:1990-10-01 00:00:00

  • The development of a real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay using TaqMan technology for the pan detection of bluetongue virus (BTV).

    abstract::Bluetongue virus (BTV) is an infectious, non-contagious viral disease of domestic and wild ruminants that is transmitted by adult females of certain Culicoides species. Since 2006, several serotypes including BTV-1, 2, 4, 6, 8, 9 and 16, have spread from the Mediterranean basin into Northern Europe for the first time....

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Mulholland C,McMenamy MJ,Hoffmann B,Earley B,Markey B,Cassidy J,Allan G,Welsh MD,McKillen J

    更新日期:2017-07-01 00:00:00

  • Assessment of template quality by the incorporation of an internal control into a RT-PCR for the detection of rabies and rabies-related viruses.

    abstract::A method is described to assess RNA template quality by the incorporation of a ribosomal RNA (rRNA) internal (in tube) control into a standard rabies and rabies-related virus specific RT-PCR. Specific virus and rRNA templates were co-amplified in a duplex reaction from RNA extracts derived from 60 isolates representin...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Smith J,McElhinney LM,Heaton PR,Black EM,Lowings JP

    更新日期:2000-02-01 00:00:00

  • Diagnosis of measles by fluorescent antibody and culture of nasopharyngeal secretions.

    abstract::An indirect fluorescent antibody test (IFA) was evaluated using commercial mouse anti-measles monoclonal antibody and FITC-labeled goat anti-mouse immunoglobulin. For measles isolation, specimens were inoculated into Rhesus monkey kidney (RMK) cells and, when available, CV-1 cells. 381 specimens were tested by IFA and...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Smaron MF,Saxon E,Wood L,McCarthy C,Morello JA

    更新日期:1991-06-01 00:00:00

  • Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum.

    abstract::The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Bhatia S,Sood R,Bhatia AK,Pattnaik B,Pradhan HK

    更新日期:2008-03-01 00:00:00

  • Evaluation of cell viability dyes in antiviral assays with RNA viruses that exhibit different cytopathogenic properties.

    abstract::Studies were conducted to determine the performance of four dyes in assessing antiviral activities of compounds against three RNA viruses with differing cytopathogenic properties. Dyes included alamarBlue® measured by absorbance (ALB-A) and fluorescence (ALB-F), neutral red (NR), Viral ToxGlo™ (VTG), and WST-1. Viruse...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Smee DF,Hurst BL,Evans WJ,Clyde N,Wright S,Peterson C,Jung KH,Day CW

    更新日期:2017-08-01 00:00:00

  • Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection.

    abstract::A recombinant murine cytomegalovirus (mCMV) that expresses enhanced green fluorescent protein (EGFP) under control of the native immediate-early 1/3 promoter was constructed to detect directly sites of viral activity in latent and reactivated infections. The recombinant virus had acute and latent infection characteris...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Henry SC,Schmader K,Brown TT,Miller SE,Howell DN,Daley GG,Hamilton JD

    更新日期:2000-09-01 00:00:00

  • A simple method for the evaluation of antiseptic and disinfectant virucidal activity.

    abstract::A simple method for evaluating the virucidal activity of water-soluble antiseptic and disinfectant products using poliovirus type 1 (Sabin strain) is described. Using a commercial concentrator, kinetic studies of four products were carried out. It was shown that 2% glutaraldehyde and 5% povidone iodine are rapidly vir...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Boudouma M,Enjalbert L,Didier J

    更新日期:1984-12-01 00:00:00

  • Detection of viruses in environmental samples: suitability of commercial rotavirus and adenovirus test kits.

    abstract::Commercially marketed kits are now available for rapid viral assay of clinical specimens. This study was conducted to determine the suitability of these kits for use in environmental testing. Eight rotavirus kits and one enteric adenovirus kit were screened for sensitivity using simian rotavirus SA11, human rotavirus ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Dahling DR,Wright BA,Williams FP Jr

    更新日期:1993-12-15 00:00:00

  • A combined genotypic and phenotypic human immunodeficiency virus type 1 recombinant virus assay for the reverse transcriptase and integrase genes.

    abstract::With the approval of the first HIV-1 integrase inhibitor raltegravir and a second one in phase III clinical development (elvitegravir), genotypic and phenotypic resistance assays are required to guide antiretroviral therapy and to investigate treatment failure. In this study, a genotypic and phenotypic recombinant vir...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Van Baelen K,Rondelez E,Van Eygen V,Ariën K,Clynhens M,Van den Zegel P,Winters B,Stuyver LJ

    更新日期:2009-11-01 00:00:00

  • WNV Typer: a server for genotyping of West Nile viruses using an alignment-free method based on a return time distribution.

    abstract::West Nile virus (WNV), genus Flavivirus, family Flaviviridae, is a major cause of viral encephalitis with broad host range and global spread. The virus has undergone a series of evolutionary changes with emergence of various genotypic lineages that are known to differ in type and severity of the diseases caused. Curre...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kolekar P,Hake N,Kale M,Kulkarni-Kale U

    更新日期:2014-03-01 00:00:00

  • RNAlater® is a viable storage option for avian influenza sampling in logistically challenging conditions.

    abstract::Surveillance of wild birds is critical in monitoring for highly pathogenic avian influenza A viruses (AIVs). However, a successful surveillance regime requires proper treatment of samples in the field - rapid placement of samples in -80°C and subsequent maintenance of cold-chain. Given the logistical difficulties of t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Wille M,Yin H,Lundkvist Å,Xu J,Muradrasoli S,Järhult JD

    更新日期:2018-02-01 00:00:00

  • Development and validation of a real-time RT-PCR assay for generic detection of pospiviroids.

    abstract::In many countries phytosanitary regulations apply to Potato spindle tuber viroid, because it can cause serious diseases in potato and tomato crops. Other pospiviroids, some of which are distributed widely in ornamental crops, can cause similar diseases. Consequently, there is a need for a reliable and cost-effective g...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Botermans M,van de Vossenberg BT,Verhoeven JT,Roenhorst JW,Hooftman M,Dekter R,Meekes ET

    更新日期:2013-01-01 00:00:00

  • Isolation of a cell line for rapid and sensitive histochemical assay for the detection of herpes simplex virus.

    abstract::A cell line which can be used in a simple, sensitive, and rapid histochemical assay was isolated for detection of herpes simplex virus (HSV). The cell line was derived by selection of G418 resistant colonies following co-transfection of baby hamster kidney cells with a plasmid which contains a G418 antibiotic resistan...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Stabell EC,Olivo PD

    更新日期:1992-08-01 00:00:00

  • In vitro replication of Macrobrachium rosenbergii nodavirus and extra small virus in C6/36 mosquito cell line.

    abstract::White tail disease (WTD) is a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India and many parts of the world. The pathogenic agents have been identified as M. rosenbergii nodavirus (MrNV) associated with extra small virus (XSV), which is 27nm and 15nm in diameter, respectively. Repli...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Sudhakaran R,Parameswaran V,Sahul Hameed AS

    更新日期:2007-12-01 00:00:00

  • A simple and rapid method for detecting human immunodeficiency virus by PCR.

    abstract::A simple, sensitive and specific method using the polymerase chain reaction (PCR) for amplification of human immunodeficiency virus type 1 (HIV-1) is described. The method involves minimal manipulations. Peripheral blood mononuclear cells (PBMC) were prepared by a rapid Ficoll-Paque gradient method. Lymphocytes were l...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Gibson KM,McLean KA,Clewley JP

    更新日期:1991-05-01 00:00:00

  • Enzymatic amplification of latent pseudorabies virus nucleic acid sequences.

    abstract::To investigate various aspects of the latency of pseudorabies virus in swine (PRV, suid herpesvirus 1) we developed in vitro nucleic acid amplification methods based upon the polymerase chain reaction. Primers flanking a 156-bp region of the pseudorabies virus gp II gene were annealed to purified PRV DNA as well as DN...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lokensgard JR,Thawley DG,Molitor TW

    更新日期:1991-09-01 00:00:00

  • In vivo mass production in the cabbage moth (Mamestra brassicae) of a heterologous (Panolis) and a homologous (Mamestra) nuclear polyhedrosis virus.

    abstract::In preparation for field trials, a nuclear polyhedrosis virus (NPV) of the pine beauty moth, Panolis flammea, was mass produced in vivo in an alternative (heterologous) host, the cabbage moth, Mamestra brassicae. Simultaneously, Mamestra NPV was also produced in M. brassicae. This homologous NPV/host system was a cons...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kelly PM,Entwistle PF

    更新日期:1988-03-01 00:00:00

  • A quantitative real-time reverse transcription PCR (qRT-PCR) assay to detect genome segment 9 of all 26 bluetongue virus serotypes.

    abstract::Bluetongue (BT) is an arboviral disease, which can often be fatal in naïve sheep and white tailed deer, but is usually less severe, or unapparent in other ruminants. Twenty-six bluetongue virus (BTV) serotypes have been recognised so far, two of which (BTV-25 and BTV-26) were recently identified by phylogenetic compar...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Maan NS,Maan S,Belaganahalli M,Pullinger G,Montes AJ,Gasparini MR,Guimera M,Nomikou K,Mertens PP

    更新日期:2015-03-01 00:00:00

  • A sensitive in-house RT-PCR genotyping system for combined detection of plasma HIV-1 and assessment of drug resistance.

    abstract::Quantification of the viral burden and identification of drug resistant mutations are important laboratory tools in the management of HIV-1 infected patients. However, widespread use of assays for viral load determination and genotyping is still hampered by the high cost. Here, an in-house RT-PCR-sequencing assay for ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Steegen K,Demecheleer E,De Cabooter N,Nges D,Temmerman M,Ndumbe P,Mandaliya K,Plum J,Verhofstede C

    更新日期:2006-05-01 00:00:00

  • A baculovirus vector derived from immediately early gene promoter of Autographa californica nuclear polyhedrosis virus.

    abstract::A transfer vector was constructed in which the neomycin resistance (neo) gene was under the control of a copy of Autographa californica nuclear polyhedrosis virus (AcMNPV) IE1 gene promoter at the p10 locus. After cotransfection of Spodoptera frugiperda (Sf9) cells with the transfer vector and infectious AcMNPV DNA, t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Zhu FX,Qi YP,Huang YX,Bing Q

    更新日期:1996-10-01 00:00:00

  • A rapid RT-LAMP assay for the detection of all four lineages of Peste des Petits Ruminants Virus.

    abstract::Peste des petits ruminants (PPR) is a viral disease of small ruminants that is caused by the PPR virus (PPRV) and is a significant burden on subsistence farmers across the developing world. Loop-mediated isothermal amplification (LAMP) provides cost-effective, rapid, specific and sensitive detection of nucleic acid an...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Rajko-Nenow P,Flannery J,Arnold H,Howson ELA,Darpel K,Stedman A,Corla A,Batten C

    更新日期:2019-12-01 00:00:00

  • Method for improving accuracy of virus titration: standardization of plaque assay for Junin virus.

    abstract::Titrating infective virus is one of the most important and common techniques in virology. However, after many years of widespread use, the parameters governing the accuracy of titration values are still not well understood. It was found that under conditions currently used for virus titration, only a small percentage ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Bushar G,Sagripanti JL

    更新日期:1990-10-01 00:00:00

  • Detection and quantitation of the new world Squash leaf curl virus by TaqMan real-time PCR.

    abstract::Squash leaf curl diseases are caused by distinct virus species that are separated into two major phylogenetic groups, western and eastern hemisphere groups. The western group includes the new world Squash leaf curl virus (SLCV) which causes major losses to cucurbit production and induces severe stunting and leaf curl ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Abrahamian PE,Abou-Jawdah Y

    更新日期:2013-07-01 00:00:00

  • Strategies for detection of transfusion-transmitted viruses eluding identification by conventional serologic tests. II. Detection of host DNA in human plasmas with elevated alanine aminotransferase.

    abstract::As a prelude to the development of nucleic acid probes specific for non-A, non-B hepatitis virus(es) (NANBV), plasmas with alanine aminotransferase levels greater than or equal to 110 IU were assayed for DNA by a radioimmunoassay. Approximately 50% of such plasmas are expected to contain NANBV. One-hundred and seventy...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Neurath AR,Strick N,Miller K,Waldman AA

    更新日期:1984-02-01 00:00:00

  • Detection of Epstein--Barr virus-determined nuclear antigen-2 mRNA by in situ hybridization.

    abstract::A mRNA in situ hybridization method was developed to detect mRNA of Epstein--Barr virus-determined nuclear antigen-2 (EBNA2). Strong in situ hybridization signals were detected in EBNA2-transfected cells with the antisense probe but not with the sense probe of this mRNA. Hybridization signals were also found in formal...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Shimakage M,Sasagawa T

    更新日期:2001-04-01 00:00:00

  • The use of polylysine during negative staining of viral suspensions.

    abstract::The use of 0.1% aqueous solution of polylysine (poly-L-lysine) is proposed as a prior step to negative staining of viral or particle suspensions. Particles spread better on films precoated with polylysine than with other substances used for the same purpose. This applies particularly to samples from sucrose or CsCl gr...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Barth OM

    更新日期:1985-05-01 00:00:00

  • Simultaneous detection of enterovirus 70 and coxsackievirus A24 variant by multiplex real-time RT-PCR using an internal control.

    abstract::Epidemics of acute hemorrhagic conjunctivitis are always explosive and extensive, and have been recognized as a serious international public health problem. Enterovirus 70 and coxsackievirus A24 variant have been identified as the major etiological agents in acute hemorrhagic conjunctivitis outbreaks worldwide. A nove...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Xiao XL,Wu H,Li YJ,Li HF,He YQ,Chen G,Zhang JW,Yang H,Li XF,Yang XQ,Yu YG

    更新日期:2009-07-01 00:00:00

  • An enzyme-immunoassay for antibodies against hepatitis B core antigen: characteristics and clinical validation.

    abstract::An enzyme-immunoassay (EIA) for antibodies to hepatitis B core antigen (anti-HBc) was developed. The new test uses undiluted samples, incubated directly into an HBcAg coated well. Three alternative test procedures are possible. The stability of reagents was studied and a preclinical evaluation was performed intramural...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Matthyssen L,Arndt-Hanser A,Lange W,Maass G,Schütt K,van Loon A,Wolters G

    更新日期:1987-08-01 00:00:00

  • Simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to PCR for human papillomavirus detection.

    abstract::Sample preparation is an important step in the detection of viral DNA by the polymerase chain reaction (PCR) technique. The method used should achieve release of cellular DNA with the minimum of manipulation steps so as to reduce the possibility of contamination. The present report demonstrates that either microwaving...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lou YK,Qin H,Molodysky E,Morris BJ

    更新日期:1993-09-01 00:00:00