Development of a recombinant yellow fever vector expressing a HIV clade C founder envelope gp120.

Abstract:

:Development of a HIV-1 vaccine is a major global priority. The yellow fever virus (YFV) attenuated vaccine 17D is among the most effective of currently used vaccines. However, the stability of the YFV17D vector when carrying non-flavivirus genes has been problematic. We have constructed and expressed HIV-1 Env in YFV17D with either single transmembrane (STM) or double transmembrane (DTM) YFV E protein domains for the development of anti-HIV antibodies. Here we describe modifications of the YFV17D vector such that HIV-1 Env gp120 is expressed in up to 5 passages in Vero cells. Immunization with recombinant YFV17D vector prime followed by HIV-1 CH505 TF gp120 protein boosts were able to induce neutralizing antibodies for a HIV-1 tier 1 isolate in mice. This modified YFV vector may be a starting point for constructing HIV-1 vaccine candidate priming vectors.

journal_name

J Virol Methods

authors

Yu JS,Liao HX,Pritchett J,Bowman C,Vivian C,Parks R,Xia SM,Cooper M,Williams WB,Bonsignori M,Reed SG,Chen M,Vandergrift N,Rice CM,Haynes BF

doi

10.1016/j.jviromet.2017.08.012

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

85-93

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(17)30414-7

journal_volume

249

pub_type

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