Abstract:
:Two enzyme-linked immunosorbent assays for detection of adenovirus antigen in faecal extracts have been established. A conjugate of rabbit anti-(human) adenovirus immunoglobulin and horseradish peroxidase (HRP) prepared by means of a hetero-bifunctional reagent, N-succinimidyl-3-(2-pyridyldithio) propionate (SPDP), was used in the conventional ELISA, and a biotin-labelled antibody in combination with a streptavidin/peroxidase complex in the other (BS-ELISA). A collection of 60 faecal extracts, in which adenovirus had been demonstrated by immunosorbent electron microscopy (ISEM) in 29 samples, was selected and examined by ELISA and BS-ELISA. The presence of adenovirus was demonstrated in 38 (63%) of the samples by BS-ELISA compared to 34 (58%) by ELISA, showing the advantage of the biotin/streptavidin system for setting up ELISA assays. Comparison of the three different techniques showed that the biotin/streptavidin (BS-ELISA) is the most sensitive assay for detection of adenovirus antigen in faecal samples.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Mortensson-Egnund K,Kjeldsberg Edoi
10.1016/0166-0934(86)90007-8subject
Has Abstractpub_date
1986-08-01 00:00:00pages
57-63issue
1eissn
0166-0934issn
1879-0984pii
0166-0934(86)90007-8journal_volume
14pub_type
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journal_title:Journal of virological methods
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