Multiplex real-time PCR for identification of canine parvovirus antigenic types.

Abstract:

:Canine parvovirus (CPV) is an important disease causing gastroenteritis and/or haemorrhagic gastroenteritis in dogs. There are four antigenic types of CPV reported worldwide viz. CPV 2, CPV 2a, CPV 2b and CPV 2c. The diagnosis of CPV with the identification of the antigen type responsible remains problematic. In the present study, identification as well as antigenic typing of CPV was done using a de novo multiplex real time PCR to combat the problem of antigenic type identification. From the study it could be concluded that the here developed multiplex real time PCR assay could be used for rapid detection of CPV as well as typing of its three antigenic types.

journal_name

J Virol Methods

authors

Kaur G,Chandra M,Dwivedi PN,Narang D

doi

10.1016/j.jviromet.2016.02.013

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

1-5

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(15)30138-5

journal_volume

233

pub_type

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