Abstract：
:Classical swine fever (CSF) is a highly contagious disease, causing severe economic losses in the pig industry worldwide. Vaccination of pigs with lapinized Chinese vaccines is still practised in some regions of the world, where the virus is enzootic, in order to prevent and control the disease. However, a single real-time assay that can detect all lapinized Chinese vaccines used widely, namely, Lapinized Philippines Coronel (LPC), Hog Cholera Lapinized virus (HCLV) and the Riems C-strain is still lacking. This study describes a real-time RT-PCR assay, targeting the N(pro) gene region, for specific detection of these lapinized vaccine strains. The assay is highly sensitive, with a detection limit of 10 genome copies per reaction for HCLV and Riems C-strain and highly specific, as more than 100 strains of wild type CSFV representing all major genotypes were not detected. The assay is also highly repeatable: the coefficient of variation of Ct values in three runs was 2.77% for the detection of 10 copies of the vaccine viral RNA. This study provides a potentially useful tool for specific detection of the lapinized Chinese vaccines, HCLV and C-strain, and the differentiation of these vaccines from wild type CSFV.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Liu L,Xia H,Everett H,Sosan O,Crooke H,Meindl-Böhmer A,Qiu HJ,Moennig V,Belák S,Widén Fdoi
10.1016/j.jviromet.2011.05.003subject
Has Abstractpub_date
2011-08-01 00:00:00pages
170-4issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(11)00199-6journal_volume
175pub_type
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