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A real time RT-PCR assay for the specific detection of Peste des petits ruminants virus.

Abstract：

:Peste des petits ruminants virus (PPRV) causes a devastating disease of small ruminants present across much of Africa and Asia. Recent surveillance activities and phylogenetic analyses have suggested that the virus is an emerging problem as it is now being detected in areas previously free of the disease. As such, the virus not only is threatening small ruminant production and agricultural stability in the developing world, but also poses an economic threat to livestock in the European Union (EU) through introduction from European Turkey and North Africa. This report describes the development of a high throughput, rapid, real time RT-PCR method for the sensitive and specific detection of PPRV using robotic RNA extraction. This assay targets the nucleocapsid (N) gene of PPRV and has been shown to detect all four genetic lineages of PPRV in tissues, ocular and nasal swabs and blood samples collected in the field. The lowest detection limit achieved was approximately 10 genome copies/reaction, making this assay an ideal tool for the sensitive and rapid detection of PPRV in diagnostic laboratories.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Batten CA,Banyard AC,King DP,Henstock MR,Edwards L,Sanders A,Buczkowski H,Oura CC,Barrett T

doi

10.1016/j.jviromet.2010.11.022

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

401-4

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(10)00421-0

journal_volume

171

pub_type

杂志文章

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