Abstract：
:No effective prophylactic or therapeutic vaccine against HIV-1 in humans is currently available. This study analyzes the immunogenicity and safety of a recombinant attenuated vaccinia virus. A chimeric gene of HIV-1 multi-epitope genes containing CpG ODN and cholera toxin B subunit (CTB) was inserted into Chinese vaccinia virus Tian Tan strain (VTT) mutant strain. The recombinant virus rddVTT(-CCMp24) was assessed for immunogenicity and safety in mice. Results showed that the protein CCMp24 was expressed stably in BHK-21 infected with rddVTT(-CCMp24). And the recombinant virus induced the production of HIV-1 p24 specific immunoglobulin G (IgG), IL-2 and IL-4. The recombinant vaccine induced γ-interferon secretion against HIV peptides, and elicited a certain levels of immunological memory. Results indicated that the recombinant virus had certain immunogenicity to HIV-1. Additionally, the virulence of the recombinant virus was been attenuated in vivo of mice compared with wild type VTT (wtVTT), and the introduction of CTB and HIV Mp24 did not alter the infectivity and virulence of defective vaccinia virus.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Du S,Wang Y,Liu C,Wang M,Zhu Y,Tan P,Ren D,Li X,Tian M,Yin R,Li C,Jin Ndoi
10.1016/j.jviromet.2015.03.007subject
Has Abstractpub_date
2015-07-01 00:00:00pages
1-9eissn
0166-0934issn
1879-0984pii
S0166-0934(15)00083-Xjournal_volume
219pub_type
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