Amplification of complete gag gene sequences from geographically distinct equine infectious anemia virus isolates.

Abstract:

:In the current study, primers described previously and modified versions of these primers were evaluated for amplification of full-length gag genes from different equine infectious anemia virus (EIAV) strains from several countries, including the USA, Germany and Japan. Each strain was inoculated into a primary horse leukocyte culture, and the full-length gag gene was amplified by reverse transcription polymerase chain reaction. Each amplified gag gene was cloned into a plasmid vector for sequencing, and the detectable copy numbers of target DNA were determined. Use of a mixture of two forward primers and one reverse primer in the polymerase chain reaction enabled the amplification of all EIAV strains used in this study. However, further study is required to confirm these primers as universal for all EIAV strains. The nucleotide sequence of gag is considered highly conserved, as evidenced by the use of gag-encoded capsid proteins as a common antigen for the detection of EIAV in serological tests. However, significant sequence variation in the gag genes of different EIAV strains was found in the current study.

journal_name

J Virol Methods

authors

Boldbaatar B,Bazartseren T,Koba R,Murakami H,Oguma K,Murakami K,Sentsui H

doi

10.1016/j.jviromet.2012.12.010

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

41-6

issue

1

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(12)00446-6

journal_volume

189

pub_type

杂志文章
  • Simultaneous detection of eight swine reproductive and respiratory pathogens using a novel GeXP analyser-based multiplex PCR assay.

    abstract::A new high-throughput GenomeLab Gene Expression Profiler (GeXP) analyser-based multiplex PCR assay was developed for the detection of eight reproductive and respiratory pathogens in swine. The reproductive and respiratory pathogens include North American porcine reproductive and respiratory syndrome virus (PRRSV-NA), ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2015.08.001

    authors: Zhang M,Xie Z,Xie L,Deng X,Xie Z,Luo S,Liu J,Pang Y,Khan MI

    更新日期:2015-11-01 00:00:00

  • TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits.

    abstract::A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the rea...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2011.11.024

    authors: Kuan CP,Huang HC,Chang CC,Lu YL

    更新日期:2012-02-01 00:00:00

  • In vitro cultivation and cryopreservation of duck embryonic hepatocytes.

    abstract::Hepatitis B-virucidal testing of biocides in quantitative suspension tests using duck hepatitis B virus (DHBV) requires primary duck embryonic hepatocytes for viral propagation. To improve the test system and availability of these cells, commercial culture plates with different growth surfaces were tested for cell cul...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2008.12.004

    authors: Schacke M,Glück B,Wutzler P,Sauerbrei A

    更新日期:2009-04-01 00:00:00

  • Analysis and genotyping of PCR products of the Amplicor HIV-1 kit.

    abstract::The Roche Amplicor PCR kit was used to detect HIV-1 DNA in UK patients of known serostatus. Four false-negative and/or equivocal results were obtained from patients who were known to be anti HIV seropositive (Tosswill et al., 1994). Cells from the blood of these patients were shown to contain HIV DNA after extraction,...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(94)00139-8

    authors: Barlow KL,Tosswill JH,Clewley JP

    更新日期:1995-03-01 00:00:00

  • Detection of Junin virus by the polymerase chain reaction.

    abstract::Argentine hemorrhagic fever is an often fatal human disease caused by Junin virus, an RNA-containing virus and member of the Arenavirus family. This virus was detected in vitro by the polymerase chain reaction (PCR) procedure. A pair of Junin virus-specific PCR DNA oligonucleotide primers and an oligonucleotide probe ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(92)90142-z

    authors: Bockstahler LE,Carney PG,Bushar G,Sagripanti JL

    更新日期:1992-09-01 00:00:00

  • Real-time PCR quantitation of hepatitis B virus total DNA and covalently closed circular DNA in peripheral blood mononuclear cells from hepatitis B virus-infected patients.

    abstract::It remains unclear whether hepatitis B virus (HBV) replicates in extrahepatic tissues, and particularly in peripheral blood mononuclear cells (PBMCs), which may serve as a reservoir for the maintenance of infection. A real-time PCR assay for the quantitation of total and covalently closed circular (ccc) HBV DNA in ser...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2006.07.019

    authors: Mazet-Wagner AA,Baclet MC,Loustaud-Ratti V,Denis F,Alain S

    更新日期:2006-12-01 00:00:00

  • Hepatitis B surface antigen polypeptide micelles from antigen expressed in Saccharomyces cerevisiae.

    abstract::Hepatitis B micelles containing the p25 component of hepatitis B surface antigen (HBsAg) have been produced by Triton X-100 solubilization followed by ultracentrifugation in linear sucrose gradients. The product was found to resemble micelle forms prepared from plasma-derived HBsAg with the surface being composed of d...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(86)90004-2

    authors: Howard CR,Young PR,Lee S,Dixon J,Zuckerman AJ,McAleer WJ,Lehman ED

    更新日期:1986-08-01 00:00:00

  • Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction.

    abstract::Universal and serotype-specific primer sets were used in simple reverse transcription polymerase chain reaction (RT-PCR) assays on field samples of epithelium and vesicular fluid to determine their suitability for primary diagnosis of all seven serotypes of foot-and-mouth disease (FMD). The specificity of reactions wa...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(00)00213-5

    authors: Reid SM,Ferris NP,Hutchings GH,Samuel AR,Knowles NJ

    更新日期:2000-09-01 00:00:00

  • Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

    abstract::Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.04.023

    authors: Shen H,Fang SG,Chen B,Chen G,Tay FP,Liu DX

    更新日期:2009-09-01 00:00:00

  • Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay.

    abstract:BACKGROUND:Influenza causes a significant annual disease burden, with characterization of the infecting virus important in clinical and public health settings. Rapid immunoassays are fast but insensitive, whereas real-time RT-PCR is sensitive but susceptible to genetic mutations and often requires multiple serial assay...

    journal_title:Journal of virological methods

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.jviromet.2019.113686

    authors: Taylor AW,Dawson ED,Blair RH,Johnson JE Jr,Slinskey AH,Smolak AW,Toth E,Liikanen K,Stoughton RS,Smith C,Talbot S,Rowlen KL

    更新日期:2019-11-01 00:00:00

  • A one-step centrifugal ultrafiltration method to concentrate enteric viruses from wastewater.

    abstract::A one-step centrifugal ultrafiltration method was developed to enhance rapid detection of human enteric viruses and co-occurring viruses in wastewater. Samples were collected pre- and post-UV treatment at two full-scale tertiary municipal wastewater treatment plants in Calgary, Canada. Viruses were concentrated from 1...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2016.09.010

    authors: Qiu Y,Lee BE,Ruecker NJ,Neumann N,Ashbolt N,Pang X

    更新日期:2016-11-01 00:00:00

  • Enterovirus genomes in wastewater: concentration on glass wool and glass powder and detection by RT-PCR.

    abstract::Standard methods for detecting enteroviruses in environmental samples require cell culture, which is time consuming and expensive. The reverse transcription-polymerase chain reaction (RT-PCR) is a rapid, sensitive method for detecting enteroviruses in water. However, environmental samples often contain substances that...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(97)02193-9

    authors: Gantzer C,Senouci S,Maul A,Levi Y,Schwartzbrod L

    更新日期:1997-05-01 00:00:00

  • Development and validation of a novel SYBR Green real-time RT-PCR assay for the detection of classical swine fever virus evaluated on different real-time PCR platforms.

    abstract::Classical swine fever is a highly contagious viral disease that causes significant economic losses in pig production on a global scale. The rapid dissemination of the virus and the variability of the clinical signs merit the development of swift and accurate classical swine fever virus (CSFV) detection methods, which ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2011.03.022

    authors: Pérez LJ,Díaz de Arce H,Tarradas J,Rosell R,Perera CL,Muñoz M,Frías MT,Nuñez JI,Ganges L

    更新日期:2011-06-01 00:00:00

  • Detection and diagnosis of parapoxvirus by the polymerase chain reaction.

    abstract::The genus Parapoxvirus includes four members, bovine papular stomatitis virus (BPSV), pseudocowpox virus (PCPV), orf virus (ORFV) and parapoxvirus of red deer in New Zealand (PVNZ). A set of primers for polymerase chain reaction (PCR) was designed to detect viral DNA from cells infected with each of the four parapoxvi...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(99)00144-5

    authors: Inoshima Y,Morooka A,Sentsui H

    更新日期:2000-02-01 00:00:00

  • Generation of a p10-based baculovirus expression vector in yeast with infectivity for insect larvae and insect cells.

    abstract::A new, versatile baculovirus vector was developed for the generation of recombinants in the yeast Saccharomyces cerevisiae and for the expression of foreign proteins in both insect larvae and in insect cells. This vector is based on Autographa californica multiple nucleocapsid nucleopolyhedrovirus (AcMNPV) and exploit...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/s0166-0934(97)00109-2

    authors: Heldens JG,Kester HA,Zuidema D,Vlak JM

    更新日期:1997-10-01 00:00:00

  • Development and laboratory evaluation of a lateral flow device for the detection of swine vesicular disease virus in clinical samples.

    abstract::A lateral flow device (LFD) for the detection of swine vesicular disease (SVD) virus (SVDV) and differential diagnosis from foot-and-mouth disease (FMD) was developed using a monoclonal antibody (Mab C70). The performance of the LFD was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.09.023

    authors: Ferris NP,Nordengrahn A,Hutchings GH,Paton DJ,Kristersson T,Merza M

    更新日期:2010-02-01 00:00:00

  • Validation of quantitative real-time RT-PCR assays for the detection of six honeybee viruses.

    abstract::Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Chronic bee paralysis virus (CBPV), Deformed wing virus (DWV), Sacbrood virus (SBV) and Varroa destructor virus 1 (VDV1) are the six main honeybee viruses reported in Europe. We assessed the accuracy (trueness and precision) of reverse transcriptase quan...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2019.04.020

    authors: Schurr F,Tison A,Militano L,Cheviron N,Sircoulomb F,Rivière MP,Ribière-Chabert M,Thiéry R,Dubois E

    更新日期:2019-08-01 00:00:00

  • Development of a Plexor real-time PCR assay for the detection of porcine circovirus type 2.

    abstract::A novel, real-time PCR system for the detection of porcine circovirus type 2 (PCV2) was developed. The system employed Plexor technology and detected 10(8)-10(1) copies per reaction of PCV2 DNA within a recombinant plasmid. The examination of clinical material showed consistent diagnostic sensitivity when samples cont...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2011.11.014

    authors: Vlasakova M,Jackova A,Leskova V,Vilcek S

    更新日期:2012-02-01 00:00:00

  • Performance of six commercial enzyme immunoassays and two alternative HIV-testing algorithms for the diagnosis of HIV-1 infection in Kisumu, Western Kenya.

    abstract::Performances of serological parallel and serial testing algorithms were analyzed using a combination of three ELISA and three rapid tests for the confirmation of HIV infection. Each was assessed individually for their sensitivity and specificity on a blinded panel of 769 retrospective sera of known HIV status. Western...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2011.05.021

    authors: Zeh C,Oyaro B,Vandenhoudt H,Amornkul P,Kasembeli A,Bondo P,Mwaengo D,Thomas TK,Hart C,Laserson KF,Ondoa P,Nkengasong JN

    更新日期:2011-09-01 00:00:00

  • Application of linker-ligation-PCR for construction of phage display epitope libraries.

    abstract::An efficient method for construction of random epitope libraries using filamentous phage is described. Random DNA fragments generated by DNase I digestion were blunt ended by T4 DNA polymerase and ligated with a 12-mer linker, followed by PCR amplification using the same oligonucleotide linker as primers. The results ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(96)02057-5

    authors: Nagesha HS,Yu M,Wang LF

    更新日期:1996-07-01 00:00:00

  • Comparison of two commercial kits and an in-house ELISA for the detection of equine rotavirus in foal feces.

    abstract::Group A rotaviruses (RVA) are important infectious agents associated with diarrhea in the young of several animal species including foals. Currently, a variety of diagnosis methods are commercially available, like ELISA, latex agglutination and immunochromatographic assays. These commercial tests are mainly designed f...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2015.05.002

    authors: Miño S,Kern A,Barrandeguy M,Parreño V

    更新日期:2015-09-15 00:00:00

  • The isolation of the two electrophoretic forms of cowpea mosaic virus using fast protein liquid chromatography.

    abstract::This report describes the first time entire viral capsids have been purified using fast protein liquid chromatography (FPLC) techniques. The FPLC is used here to separate the two electrophoretic forms of cowpea mosaic virus. The capsid forms are shown to be separated by the Mono-Q column without damaging the capsids. ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(87)90011-5

    authors: Smith TJ

    更新日期:1987-07-01 00:00:00

  • Molecular detection of varicella zoster virus while keeping an eye on the budget.

    abstract::Varicella zoster virus (VZV) PCR is highly sensitive compared to traditional detection methods like culture and direct fluorescent antibody testing (DFA); however, the high cost of commercial assays prohibits their use in many clinical laboratories. Major contributors to cost are the nucleic acid extraction and the PC...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2014.02.009

    authors: Binkhamis K,Al-Siyabi T,Heinstein C,Hatchette TF,LeBlanc JJ

    更新日期:2014-06-01 00:00:00

  • Expression and purification of bioactive hemagglutinin protein of highly pathogenic avian influenza A (H5N1) in silkworm larvae.

    abstract::The hemagglutinin (HA) of avian influenza viruses plays a very important role in the infection of host cells. In this study, the HA gene of the highly pathogenic avian influenza H5N1 virus was cloned and expressed in silkworm larvae. The expressed recombinant HA (rHA) was purified using fetuin-agarose chromatography a...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2013.08.040

    authors: Dong J,Harada M,Yoshida S,Kato Y,Murakawa A,Ogata M,Kato T,Usui T,Park EY

    更新日期:2013-12-01 00:00:00

  • Pan-serotypic detection of foot-and-mouth disease virus using a minor groove binder probe reverse transcription polymerase chain reaction assay.

    abstract::A novel assay for the pan-serotypic detection of foot-and-mouth disease virus (FMDV) was designed using a 5' conjugated minor groove binder (MGB) probe real-time RT-PCR system. This assay targets the 3D region of the FMDV genome and is capable of detecting 20 copies of a transcribed RNA standard. The linear range of t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2011.03.008

    authors: McKillen J,McMenamy M,Reid SM,Duffy C,Hjertner B,King DP,Bélak S,Welsh M,Allan G

    更新日期:2011-06-01 00:00:00

  • Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains.

    abstract::A real-time RT-PCR assay based on the TaqMan technology was developed for rapid and sensitive detection of pestiviruses infecting cattle, i.e., bovine viral diarrhea virus (BVDV) 1, BVDV-2, and the emerging HoBi-like pestiviruses. The assay was linear and reproducible, being able to detect as few as 10 copies of viral...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2015.08.013

    authors: Losurdo M,Mari V,Lucente MS,Colaianni ML,Padalino I,Cavaliere N,Buonavoglia C,Decaro N

    更新日期:2015-11-01 00:00:00

  • A method for the purification of rotaviruses and adenoviruses from faeces.

    abstract::Rotaviruses and non-cultivable enteric adenoviruses were purified from large volumes of faecal extracts using polyethylene glycol precipitation, centrifugation through 45% w/v sucrose and segregation by density in a cesium chloride density gradient. Yields were high and the preparations were pure enough to use in the ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(82)90059-3

    authors: Beards GM

    更新日期:1982-08-01 00:00:00

  • Method for improving accuracy of virus titration: standardization of plaque assay for Junin virus.

    abstract::Titrating infective virus is one of the most important and common techniques in virology. However, after many years of widespread use, the parameters governing the accuracy of titration values are still not well understood. It was found that under conditions currently used for virus titration, only a small percentage ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/0166-0934(90)90047-j

    authors: Bushar G,Sagripanti JL

    更新日期:1990-10-01 00:00:00

  • High-quality virus images obtained by transmission electron microscopy and charge coupled device digital camera technology.

    abstract::The introduction of digital cameras has led to the publication of numerous virus electron micrographs of low magnification, poor contrast, and low resolution. Described herein is the methodology for obtaining highly contrasted virus images in the magnification range of approximately 250,000-300,000x. Based on recent a...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2009.03.004

    authors: Tiekotter KL,Ackermann HW

    更新日期:2009-07-01 00:00:00

  • Simple and rapid strategy for genetic characterization of influenza B virus reassortants.

    abstract::Genetic reassortment of influenza viruses is widely used for creating viruses with specific phenotypes. Reassortment of two influenza viruses, each with eight RNA segments potentially yields as many as 256 gene segment combinations. Therefore, confirmation that progeny viruses possess genomes corresponding to the spec...

    journal_title:Journal of virological methods

    pub_type: 杂志文章

    doi:10.1016/j.jviromet.2004.11.024

    authors: Lugovtsev VY,Vodeiko GM,Strupczewski CM,Levandowski RA

    更新日期:2005-03-01 00:00:00