Abstract:
:A Western blot assay was developed and analyzed against the comparable standard, immunoprecipitation of (35)[S]-methionine/cysteine-labeled ovine progressive pneumonia virus (OPPV) proteins, for its ability to detect anti-OPPV antibodies using endpoint titers. Western blot analysis is 12-fold more sensitive in detecting endpoint anti-capsid antibody titers than IP, and the capsid is the B-cell immunodominant OPPV protein when utilizing Western blot analysis. Since the surface envelope glycoprotein is the B-cell immunodominant OPPV protein when utilizing immunoprecipitation, this suggests immunoprecipitation and Western blot analysis measure different types of antibody that are more specific for conformational and linear OPPV protein epitopes, respectively.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Myers-Evert DK,Herrmann-Hoesing LMdoi
10.1016/j.jviromet.2006.06.025subject
Has Abstractpub_date
2006-11-01 00:00:00pages
339-42issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(06)00237-0journal_volume
137pub_type
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