当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Development of a real-time reverse transcription polymerase chain reaction assay for detection of marine caliciviruses (genus Vesivirus).

Development of a real-time reverse transcription polymerase chain reaction assay for detection of marine caliciviruses (genus Vesivirus).

Abstract：

:Marine caliciviruses form a distinct lineage within the genus Vesivirus (family Caliciviridae). This group includes vesicular exanthema of swine virus (VESV) and San Miguel sea lion virus (SMSV) and other related viruses which have been proposed to be marine in origin isolated from a variety of terrestrial and marine animals. Rapid and reliable detection of marine caliciviruses is important as these viruses appear to be widespread and can cause vesicular disease in a wide variety of susceptible hosts including pigs and experimentally infected cattle where clinical signs cannot be easily distinguished from foot-and-mouth disease (FMD), swine vesicular disease (SVD) and vesicular stomatitis (VS). A real-time RT-PCR assay targeting conserved nucleotide sequences in the RNA-dependent RNA polymerase (3D) region of the genome successfully detected cell culture-grown virus preparations of more than thirty marine calicivirus serotypes. Only the atypical SMSV serotypes 8 and 12 failed to be detected, which provided further indication of genetic divergence between these and the other calicivirus serotypes said to be marine in origin. The real-time RT-PCR assay also specifically amplified RNA from samples collected following experimental inoculation of pigs with VESV. No cross-reactivity was demonstrated when the assay was tested with RNA prepared from representative viruses of FMD, SVD and VS. The real-time RT-PCR assay described is a sensitive and specific tool for detection and differential diagnosis of these viruses from other vesicular-disease causing viruses.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Reid SM,King DP,Shaw AE,Knowles NJ,Hutchings GH,Cooper EJ,Smith AW,Ferris NP

doi

10.1016/j.jviromet.2006.11.010

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

166-73

issue

1-2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(06)00398-3

journal_volume

140

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • Characterization of molecular clones of porcine endogenous retrovirus-A containing different numbers of U3 repeat boxes in the long terminal repeat region.

    abstract：:When full-length molecular clones of porcine endogenous retrovirus (PERV)-A and porcine endogenous retrovirus (PERV)-B were passaged on human cells, an increase in the length of the long terminal repeat (LTR) was reported. A 39-bp repeat box in the LTR U3 region was multimerized dynamically upon replication, acting as...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.01.023

    authors： Lee YJ,Park SH,Bae EH,Jung YT

    更新日期：2012-04-01 00:00:00

  • Structural and biological properties of Cucumber mosaic virus particles carrying hepatitis C virus-derived epitopes.

    abstract：:The Cucumber mosaic virus (CMV) is a three-component isodiametric plant virus with an extremely wide host range, present worldwide. A pseudorecombinant form has been described, deriving from the RNA3 component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA 1, 2 components of the CMV-D strain. Th...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.10.005

    authors： Nuzzaci M,Bochicchio I,De Stradis A,Vitti A,Natilla A,Piazzolla P,Tamburro AM

    更新日期：2009-02-01 00:00:00

  • New Coxsackievirus 2Apro and 3Cpro protease antibodies for virus detection and discovery of pathogenic mechanisms.

    abstract：:Enteroviruses (EVs), such as the Coxsackie B-viruses (CVBs), are common human pathogens, which can cause severe diseases including meningitis, myocarditis and neonatal sepsis. EVs encode two proteases (2Apro and 3Cpro), which perform the proteolytic cleavage of the CVB polyprotein and also cleave host cell proteins to...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2018.02.001

    authors： Laitinen OH,Svedin E,Kapell S,Hankaniemi MM,Larsson PG,Domsgen E,Stone VM,Määttä JAE,Hyöty H,Hytönen VP,Flodström-Tullberg M

    更新日期：2018-05-01 00:00:00

  • Differential expression of two isolates of beak and feather disease virus capsid protein in Escherichia coli.

    abstract：:Expression of recombinant beak and feather disease virus (BFDV) capsid-associated protein (Cap) has relied on inefficient techniques that typically produce low yields or use specialized expression systems, which greatly increase the cost and expertise required for mass production. An Escherichia coli system was used t...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.01.020

    authors： Patterson EI,Swarbrick CM,Roman N,Forwood JK,Raidal SR

    更新日期：2013-04-01 00:00:00

  • Suitability of autoclaved tap water for preparation of ELISA reagents and washing buffer.

    abstract：:The suitability of autoclaved tap water for the preparation of ELISA reagents and washing buffer was compared with that of ultrapure water, in a standard indirect ELISA for the detection of antibodies to pseudorabies virus (PRV). The performance of the assay, using autoclaved tap water (AT-ELISA) compared favourably t...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(94)90110-4

    authors： Afshar A,Zhao X,Heckert RA,Trotter HC

    更新日期：1994-02-01 00:00:00

  • Evaluation of an incubation instrument-free reverse transcription recombinase polymerase amplification assay for rapid and point-of-need detection of canine distemper virus.

    abstract：:Canine distemper, caused by Canine distemper virus (CDV), is a highly contagious and fatal systemic disease in free-living and captive carnivores worldwide. Accurate, rapid and simple detection of CDV is critical to improve disease management and prevent outbreaks. In this study, a visible and incubation instrument-fr...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2018.07.007

    authors： Wang J,Wang J,Li R,Shi R,Liu L,Yuan W

    更新日期：2018-10-01 00:00:00

  • Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system.

    abstract：:A quantitative and simple indirect radioimmunoassay (IRIA) was developed for porcine parvovirus (PPV), employing a monoclonal antibody directed against PPV adsorbed to nitrocellulose membrane. The IRIA was equally sensitive to live or inactivated PPV. There was a linear relationship between membrane-bound radioactivit...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(85)90129-6

    authors： Katz JB,van Deusen RA

    更新日期：1985-12-01 00:00:00

  • Proteasome inhibition reduces avian reovirus replication and apoptosis induction in cultured cells.

    abstract：:The interplay between avian reovirus (ARV) replication and apoptosis and proteasome pathway was studied in cultured cells. It is shown that inhibition of the proteasome did not affect viral entry and host cell translation but had influence on ARV replication and ARV-induced apoptosis. Evidence is provided to demonstra...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.03.016

    authors： Chen YT,Lin CH,Ji WT,Li SK,Liu HJ

    更新日期：2008-07-01 00:00:00

  • Development of a universal RT-PCR for amplifying and sequencing the leader and capsid-coding region of foot-and-mouth disease virus.

    abstract：:Foot-and-mouth disease (FMD) is a highly infectious viral disease of cloven-hoofed animals with debilitating and devastating consequences for livestock industries throughout the world. Key antigenic determinants of the causative agent, FMD virus (FMDV), reside within the surface-exposed proteins of the viral capsid. T...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.01.009

    authors： Xu L,Hurtle W,Rowland JM,Casteran KA,Bucko SM,Grau FR,Valdazo-González B,Knowles NJ,King DP,Beckham TR,McIntosh MT

    更新日期：2013-04-01 00:00:00

  • Rapid detection of giant salamander iridovirus by cross-priming amplification.

    abstract：:Giant salamander iridovirus (GSIV) belongs to the epizootic genus Ranavirus, and is the cause of epidemic diseases associated with high mortality and great losses to artificial breeding and farming. Here, we established a simple, accurate, and reliable cross-priming amplification (CPA) method to detect GSIV. The CPA a...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2019.113678

    authors： Zhang H,Wang F,Jia R,Fan J,Hao J,Du J,Niu Y,Han S,Deng R,Zhang G

    更新日期：2019-12-01 00:00:00

  • Secreted expression of the classical swine fever virus glycoprotein E(rns) in yeast and application to a sandwich blocking ELISA.

    abstract：:E(rns) is an envelope glycoprotein of classical swine fever virus (CSFV) with RNase activity. The purpose of this study was to produce an active E(rns) for further applications using the yeast secreted expression system. The E(rns) gene was cloned into the expression vector pGAPZalphaC which was introduced into Pichia...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.08.020

    authors： Huang C,Chien MS,Hu CM,Chen CW,Hsieh PC

    更新日期：2006-03-01 00:00:00

  • Application of shRNA-containing herpes simplex virus type 1 (HSV-1)-based gene therapy for HSV-2-induced genital herpes.

    abstract：:HSV-1-based vectors have been widely used to achieve targeted delivery of genes into the nervous system. In the current study, we aim to use shRNA-containing HSV-1-based gene delivery system for the therapy of HSV-2 infection. Guinea pigs were infected intravaginally with HSV-2 and scored daily for 100 days for the se...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.06.037

    authors： Liu Z,Xiang Y,Wei Z,Yu B,Shao Y,Zhang J,Yang H,Li M,Guan M,Wan J,Zhang W

    更新日期：2013-11-01 00:00:00

  • The selection and characterization of human monoclonal antibodies to human cytomegalovirus.

    abstract：:This communication describes the application of Epstein-Barr virus lymphocyte transformation technology to the production of human monoclonal antibodies specific for human cytomegalovirus. A group of such IgG antibodies have been characterized in terms of subclass, light-chain composition, specificity for particular v...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(86)90003-0

    authors： Redmond MJ,Leyritz-Wills M,Winger L,Scraba DG

    更新日期：1986-08-01 00:00:00

  • Sensitive detection of nucleic acids and protein of human papillomavirus type 6 in respiratory and genital tract papillomata.

    abstract：:We have developed a sensitive method to detect and localize HPV-6 viral DNA, mRNA and protein in biopsy specimens of genital and respiratory tract lesions by using in situ hybridization and immunoperoxidase assays on sections of plastic-embedded tissue. This modified in situ hybridization technique, using ultrathin se...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(89)90098-0

    authors： Wu TC,Mounts P

    更新日期：1989-07-01 00:00:00

  • A simplified cloning strategy for the generation of an endothelial cell selective recombinant adenovirus vector.

    abstract：:Specifically targeting adenoviral vectors to particular cell/tissue types can be achieved by genetically modifying the adenovirus fiber protein. Two common strategies are: (1) directly modifying the fiber gene in the adenovirus genome and (2) in trans supply of the modified fiber. The former however, suffers from diff...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2006.02.010

    authors： Mahanivong C,Krüger JA,Bian D,Reisfeld RA,Huang S

    更新日期：2006-07-01 00:00:00

  • A comparison between one first generation and three second generation anti-HCV ELISAs: an investigation in high- and low-risk subjects in correlation with recombinant immunoblot assay and polymerase chain reaction.

    abstract：:One first generation assay (manufactured by Ortho, test I) and 3 second generation anti-HCV ELISAs (manufactured by Ortho, Abbott, and UBI, tests II-IV) were compared. Sera from 4 different sources were used: (1) intravenous drug-users (IVDUs, n = 50), (2) blood donors (n = 1055), (3) all clinical samples from one day...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(92)90087-t

    authors： Bååth L,Widell A,Nordenfelt E

    更新日期：1992-12-01 00:00:00

  • A quantitative assay for measuring human foamy virus using an established indicator cell line.

    abstract：:In order to improve the accuracy for detecting human foamy virus (HFV), an indicator cell line was established by co-transfecting baby hamster kidney-21 cells with two plasmids: one containing a G418 antibiotic resistance marker and the other including the luc gene which was placed downstream of the inducible HFV long...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00285-3

    authors： Tai HY,Sun KH,Kung SH,Liu WT

    更新日期：2001-05-01 00:00:00

  • Multiplex polymerase chain reaction for the simultaneous detection and typing of polyomavirus JC, BK and SV40 DNA in clinical samples.

    abstract：:A novel multiplex nested PCR (nPCR) method was developed for detecting and differentiating simultaneously the DNA of polyomaviruses JC, BK and SV40 in a single tube. In the first amplification step the same set of primers were used to amplify a conserved DNA region of the large T antigen gene of JCV, BKV and SV40. The...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(99)00095-6

    authors： Fedele CG,Ciardi M,Delia S,Echevarria JM,Tenorio A

    更新日期：1999-10-01 00:00:00

  • Protamine sulfate as an effective alternative to polybrene in retroviral-mediated gene-transfer: implications for human gene therapy.

    abstract：:The polycation protamine sulfate was compared to polybrene, the usual agent employed, for its ability to increase the efficiency of retroviral infection. The murine retroviral vector SAX, which contains the neoR gene and the human adenosine deaminase (ADA) cDNA, was used as a marker of cell infection. SAX viral supern...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(89)90132-8

    authors： Cornetta K,Anderson WF

    更新日期：1989-02-01 00:00:00

  • Detection and quantitation of the new world Squash leaf curl virus by TaqMan real-time PCR.

    abstract：:Squash leaf curl diseases are caused by distinct virus species that are separated into two major phylogenetic groups, western and eastern hemisphere groups. The western group includes the new world Squash leaf curl virus (SLCV) which causes major losses to cucurbit production and induces severe stunting and leaf curl ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.04.001

    authors： Abrahamian PE,Abou-Jawdah Y

    更新日期：2013-07-01 00:00:00

  • Novel post-translational modifications of the hemagglutinin and neuraminidase proteins of avian influenza virus expressed by Kluyveromyces lactis.

    abstract：:Avian influenza virus (AIV) is an enveloped virus with segmented RNA that belongs to the Orthomyxoviridae. Recently, avian influenza virus isolates have not only posed a significant threat to the poultry industry but also serious public health concerns. The full-length viral hemagglutinin (HA), neuraminidase (NA) or b...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.05.006

    authors： Tsai SM,Chiang YC,Chin LT,Liu HJ,Wang CY

    更新日期：2011-08-01 00:00:00

  • Development and optimization of plaque assays for rat coronaviruses.

    abstract：:Plaque assays under Sephadex or agarose overlays are described for rat coronaviruses (RCVs) grown in L2 mouse fibroblasts. A plaque assay using Sephadex was simple; however, viable plaques could not be collected for propagation, and fixation was necessary before evaluation. Plaque formation under agarose was optimized...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(93)90089-a

    authors： Gaertner DJ,Winograd DF,Compton SR,Paturzo FX,Smith AL

    更新日期：1993-06-01 00:00:00

  • Coupling of foot-and-mouth disease virus to sheep red blood cells using tannic acid for immunological assays.

    abstract：:A technique for coupling foot-and-mouth disease virus (FMDV) to tanned sheep red blood cells (SRBC) is reported. Different parameters influencing the procedure were studied. Subtypes C2, C3, O1 and A24 were used as antigens, and guinea pig hyperimmune sera obtained were tested for specific antibody in passive hemagglu...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(88)90131-0

    authors： Pujol CA,Feledi CA,Massouh EJ

    更新日期：1988-08-01 00:00:00

  • A method for increasing the surface details of resin embedded viruses using Congo/Crimean haemorrhagic fever virus as a model.

    abstract：:The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(84)90040-5

    authors： Ellis DS,Smith MD,Lloyd G,Bowen ET,Simpson DI

    更新日期：1984-02-01 00:00:00

  • Development of a quantitative enzyme linked immunosorbent assay for monitoring the Enterovirus 71 vaccine manufacturing process.

    abstract：:Enterovirus 71 (EV71), the etiologic agent causes outbreaks with significant mortality in young children in Asia and currently there is no vaccine available. In this study, we report a quantitative enzyme linked immunosorbent assay (Q-ELISA) to determine the concentration of the EV71 VP2 antigen. EV71 virus-like parti...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.06.001

    authors： Liu CC,Chang HW,Yang G,Chiang JR,Chow YH,Sai IH,Chang JY,Lin SC,Sia C,Hsiao CH,Chou AH,Chong P

    更新日期：2011-09-01 00:00:00

  • The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting rotavirus A and norovirus.

    abstract：:Rotavirus (RoV) and Norovirus (NoV) are the main causes of viral gastroenteritis. Currently, there is no validated multiplex real-time PCR that can detect and quantify RoV and NoV simultaneously. The aim of the study was to develop, validate, and internally control a multiplex one-step RT real-time PCR to detect and q...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.09.021

    authors： Dung TT,Phat VV,Nga TV,My PV,Duy PT,Campbell JI,Thuy CT,Hoang NV,Van Minh P,Le Phuc H,Tuyet PT,Vinh H,Kien DT,Huy Hle A,Vinh NT,Nga TT,Hau NT,Chinh NT,Thuong TC,Tuan HM,Simmons C,Farrar JJ,Baker S

    更新日期：2013-01-01 00:00:00

  • Screening of PRRSV- and ASFV-encoded proteins involved in the inflammatory response using a porcine iGLuc reporter.

    abstract：:Inflammasome plays a major role in innate immune responses by activating caspase-1, resulting in secretion of interleukin-1β (IL-1β) and inflammatory pathologic responses. IL-1β release is widely used as an indirect readout to study inflammasome activation. Here we report an iGLuc reporter (pro-IL-1β-Gluc) of pig orig...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2020.113958

    authors： Song J,Li K,Li T,Zhao G,Zhou S,Li H,Li J,Weng C

    更新日期：2020-11-01 00:00:00

  • An inhibition enzyme-linked immunosorbent assay for the detection of antibody to Rift Valley fever virus in humans, domestic and wild ruminants.

    abstract：:This paper describes the development and validation of an inhibition ELISA based on gamma-irradiated tissue culture-derived antigen for the detection of antibody to Rift Valley fever virus (RVFV) in humans, domestic and wild ruminants. Validation data sets derived from field-collected sera in Africa (humans=1367, catt...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.02.008

    authors： Paweska JT,Mortimer E,Leman PA,Swanepoel R

    更新日期：2005-07-01 00:00:00

  • Coupling generation of cytomegalovirus deletion mutants and amplification of viral BAC clones.

    abstract：:Human cytomegalovirus (HCMV) genome manipulation has always been difficult. Recently, the introduction of full-length HCMV DNA into Escherichia coli as an artificial bacterial chromosome (BAC) clone has allowed reliable targeted mutagenesis. Here, we show the next generation of improvement in designing recombinant HCM...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2004.06.009

    authors： Wang W,Patterson CE,Yang S,Zhu H

    更新日期：2004-11-01 00:00:00

  • Real-time RT-PCR differentiation and quantitation of infectious bursal disease virus strains using dual-labeled fluorescent probes.

    abstract：:A real-time RT-PCR assay was developed utilizing dual-labeled fluorescent probes binding to VP4 sequence that are specific to the classical (Cl), variant (V) and very virulent (vv) strains of infectious bursal disease virus (IBDV). The assay was highly sensitive and could detect as little as 3 x 10(2) to 3 x 10(3) cop...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2005.03.009

    authors： Peters MA,Lin TL,Wu CC

    更新日期：2005-07-01 00:00:00