Abstract：
:In the context of eradication of poliomyelitis the World Health Organization stimulates the development of inactivated polio vaccines based on attenuated virus strains. In addition to vaccine development, tests have to be designed to assess the vaccine quality. An important test is the identification test for poliovirus strains that are used for the vaccine production. A rapid and accurate PCR method with fluorescent probes has been developed to identify unequivocally the vaccine-specific poliovirus strains, such as Mahoney, MEF-1, Saukett H, Sabin type 1, Sabin type 2 and Sabin type 3.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Nijst OE,Mouthaan JJ,Mekkes DR,Jusic E,van der Avoort HG,Metz Bdoi
10.1016/j.jviromet.2013.01.026subject
Has Abstractpub_date
2013-04-01 00:00:00pages
189-95issue
1eissn
0166-0934issn
1879-0984pii
S0166-0934(13)00051-7journal_volume
189pub_type
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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abstract：:Detection of classical swine fever virus (CSFV) and its discrimination from other pestiviruses can be achieved by virus isolation (VI) in cell cultures, antigen detection, or molecular analysis. To simplify the latter, a 5'-nuclease assay (TaqMan) was developed for the rapid and specific detection of CSFV with the min...
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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doi：10.1016/0166-0934(92)90063-j
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