Abstract:
:Herpes simplex virus (HSV) isolates were differentiated by immunoblotting analysis using a mixture of polyclonal antisera directed against HSV type 1 (HSV-1) and HSV type 2 (HSV-2) glycoprotein fractions (gB/gC of HSV-1 and gC/gE/84-kDa protein of HSV-2), since the mixed antisera recognized viral polypeptides with different molecular weights in HSV-1- and HSV-2-infected cells. Results of typing by immunoblotting analysis were consistent with those obtained by restriction endonuclease analysis of DNAs extracted from 10 HSV isolates. These results suggest that the immunoblotting technique will be applicable to reliable typing of HSV isolates using polyclonal antisera showing the difference in reaction patterns between HSV-1- and HSV-2-infected cells.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Okazaki K,Chiya S,Ideguchi S,Kimura Sdoi
10.1016/0166-0934(87)90122-4subject
Has Abstractpub_date
1987-11-01 00:00:00pages
169-78issue
2-3eissn
0166-0934issn
1879-0984pii
0166-0934(87)90122-4journal_volume
18pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2020-10-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
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doi:10.1016/j.jviromet.2008.02.005
更新日期:2008-05-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/j.jviromet.2009.02.003
更新日期:2009-10-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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更新日期:2002-08-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(90)90133-z
更新日期:1990-02-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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