Abstract:
:Hepatitis B micelles containing the p25 component of hepatitis B surface antigen (HBsAg) have been produced by Triton X-100 solubilization followed by ultracentrifugation in linear sucrose gradients. The product was found to resemble micelle forms prepared from plasma-derived HBsAg with the surface being composed of discrete globular and stranded sub-units. The degree of immunochemical relatedness of the micellular preparation was compared to the native 22-nm HBsAg particle present in either plasma or yeast cell extracts. The yeast micelle preparation competed for anti-HBs in a similar manner as intact HBsAg of plasma origin. Enhanced immunogenicity may be expected for micelles containing a recombinant HBsAg protein as has previously been shown for the plasma-derived antigen.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Howard CR,Young PR,Lee S,Dixon J,Zuckerman AJ,McAleer WJ,Lehman EDdoi
10.1016/0166-0934(86)90004-2subject
Has Abstractpub_date
1986-08-01 00:00:00pages
25-35issue
1eissn
0166-0934issn
1879-0984pii
0166-0934(86)90004-2journal_volume
14pub_type
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