Abstract：
:Two fragments, S66 and S55, of the S glycoprotein of the newly identified canine coronavirus type I (CCoV type I), were expressed in a procariotic system. The purified recombinant proteins of 350 and 366 amino acids in length, respectively, were employed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of CCoV type I antibodies in dog sera. Four canine sera-positive for CCoV type II, four sera-positive for CCoV type I and 10 negative control sera were examined. Only the sera-positive for CCoV type I strongly reacted with both the proteins, whereas the sera-positive for CCoV type II showed low reactivity in the ELISA test. As CCoV type I seems to be not cultivable in cell cultures, the recombinant fragments of the S protein represent a unique method to study, preliminarily, the immunological and the pathogenetic characteristics of this new virus.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Pratelli A,Elia G,Decaro N,Tola S,Tinelli A,Martella V,Rocca S,Tempesta M,Buonavoglia Cdoi
10.1016/j.jviromet.2003.12.003subject
Has Abstractpub_date
2004-04-01 00:00:00pages
61-5issue
1eissn
0166-0934issn
1879-0984pii
S0166093403004014journal_volume
117pub_type
杂志文章abstract：:A novel assay for the pan-serotypic detection of foot-and-mouth disease virus (FMDV) was designed using a 5' conjugated minor groove binder (MGB) probe real-time RT-PCR system. This assay targets the 3D region of the FMDV genome and is capable of detecting 20 copies of a transcribed RNA standard. The linear range of t...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2001-09-01 00:00:00
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