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Detection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material.

Abstract：

:The latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-wild type LMP1 and compared the presence of the antibody staining with LMP1 DNA sequence analysis on clinical samples of nasopharyngeal carcinoma, peripheral T-cell lymphoma (PTCL), Hodgkin's disease, lymphoblastoid cell lines (LCL) from normal volunteers, and patients with nasopharyngeal carcinoma. The results demonstrate specificity of the monoclonal cocktail for detecting the non-wild type LMP1 and the ability to sub-differentiate between the mediterranean type of LMP1 and the CAO-LMP1. Double immunofluorescence on paraffin material using the traditional CS1-4 monoclonal antibodies and the CAO-cocktail revealed no dual population of cells in the biopsy material from the Asian region.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Nicholls J,Hahn P,Kremmer E,Fröhlich T,Arnold GJ,Sham J,Kwong D,Grässer FA

doi

10.1016/j.jviromet.2003.10.015

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

79-88

issue

1

eissn

0166-0934

issn

1879-0984

pii

S0166093403003549

journal_volume

116

pub_type

杂志文章

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