Immortalized porcine intestinal epithelial cell cultures susceptible to porcine rotavirus infection.


:In vitro studies related to various viral pathogenesis in swine have been hampered by the lack of relevant porcine cell lines. The susceptibility to porcine rotavirus infection was evaluated by using a newly established porcine intestinal epithelial cell line. Immunohistochemical staining for cytokeratin confirmed that the cultured cells were epithelial cells. Measurement of cell viability and detection of infected cells confirmed that these epithelial cells were susceptible to porcine rotavirus infection. This study describes the cytopathic changes in cultured porcine intestinal epithelial cells during virus invasion. Following infection with porcine rotavirus, the cell cultures contained viral protein at 16 h post-infection as detected by direct immunofluorescence. The epithelial cell cultures provided competent target cells for studying host cell responses to porcine rotavirus and a homologous system for investigating the response of intestinal epithelial cells during viral infection.


J Virol Methods


Wang J,Hu G,Gao W,Xu L,Ning P,Zhang Y




Has Abstract


2014-06-01 00:00:00












  • A simple and rapid method for detecting human immunodeficiency virus by PCR.

    abstract::A simple, sensitive and specific method using the polymerase chain reaction (PCR) for amplification of human immunodeficiency virus type 1 (HIV-1) is described. The method involves minimal manipulations. Peripheral blood mononuclear cells (PBMC) were prepared by a rapid Ficoll-Paque gradient method. Lymphocytes were l...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Gibson KM,McLean KA,Clewley JP

    更新日期:1991-05-01 00:00:00

  • Detection of human papillomavirus DNA and mRNA using synthetic, type-specific oligonucleotide probes.

    abstract::Type-specific 30'mer-36'mer oligonucleotide probes complementary to mRNA transcribed from the E6 and E7 open reading frames of HPV 6b/11, 16, 18 and 33 were designed using the published nucleotide sequences. As oligonucleotides are easily and relatively cheaply synthesized in large amounts and are free of vector DNA, ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Faulkner-Jones BE,Tabrizi SN,Borg AJ,Roche PJ,Haralambidis J,Coghlan JP,Garland SM

    更新日期:1993-03-01 00:00:00

  • Detection of CMV in urine: comparison between DNA-DNA hybridization, virus isolation, and immunoelectron microscopy.

    abstract::Rapid detection of CMV-DNA in urine specimens by dot-blot hybridization was compared to conventional virus isolation and to virus identification using solid-phase immunoelectron microscopy (SPIEM). To detect viral DNA, 32P-labeled EcoR1 J fragment of CMV-DNA was used as a probe in the hybridization assay. In addition,...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Vonsover A,Gotlieb-Stematsky T,Sayar Y,Bardov L,Manor Y,Siegal B

    更新日期:1987-05-01 00:00:00

  • Demonstration of calicivirus in human faeces by immunosorbent and immunogold-labelling electron microscopy methods.

    abstract::Immunosorbent electron microscopy (ISEM) and immuno-gold staining (IGS) electron microscopy methods have been applied to human faeces, shown by direct electron microscopy (EM) to contain calicivirus. Caliciviruses were successfully trapped on grids coated with positive rabbit or human antisera against calicivirus, but...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kjeldsberg E

    更新日期:1986-11-01 00:00:00

  • Inoculation of plants with begomoviruses by particle bombardment without cloning: Using rolling circle amplification of total DNA from infected plants and whiteflies.

    abstract::A new system for inoculation of plants with begomoviral DNA without cloning or the use insect vectors is described. Total DNA extracted from begomovirus-infected plants was amplified by rolling circle amplification (RCA) using the bacteriophage phi29 DNA polymerase, and inoculated to plants by particle bombardment. In...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Guenoune-Gelbart D,Sufrin-Ringwald T,Capobianco H,Gaba V,Polston JE,Lapidot M

    更新日期:2010-09-01 00:00:00

  • Simultaneous detection of six stone fruit viruses by non-isotopic molecular hybridization using a unique riboprobe or 'polyprobe'.

    abstract::A new strategy for the simultaneous detection of plant viruses by molecular hybridization has been developed. Two, four or six viral sequences were fused in tandem and transcribed to render unique riboprobes and designated as 'polyprobes'. The 'polyprobe four' (poly 4) covered the four ilarviruses affecting stone frui...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Herranz MC,Sanchez-Navarro JA,Aparicio F,Pallás V

    更新日期:2005-03-01 00:00:00

  • Use of internal standard RNA molecules for the RT-PCR amplification of the faeces-borne RNA viruses.

    abstract::The diagnostic system based on reverse transcription (RT)-PCR has been used widely for the detection of viral genomes of faecal-borne RNA viruses. However, faecal specimens often produce both false positive and false negative results. Therefore, there is a need for a diagnosis procedure that can control for 'false-res...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kim K,Park J,Chung Y,Cheon D,Lee IB,Lee S,Yoon J,Cho H,Song C,Lee KH

    更新日期:2002-07-01 00:00:00

  • Comparison of PCR primer pairs in the detection of human rhinoviruses in nasopharyngeal aspirates.

    abstract::The development of a rapid and highly sensitive PCR assay for the detection of human rhinoviruses (HRVs) in nasopharyngeal aspirates is described. Two simple and fast commercial RNA extraction methods and four primer pairs were compared. The most sensitive RNA extraction method (Ultraspec) and primer pair (A) were app...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Santti J,Hyypiä T,Halonen P

    更新日期:1997-06-01 00:00:00

  • The creation of stable cell lines expressing Ebola virus glycoproteins and the matrix protein VP40 and generating Ebola virus-like particles utilizing an ecdysone inducible mammalian expression system.

    abstract::Ebola virus is a filovirus that causes hemorrhagic fever in humans and is associated with case fatality rates of up to 90%. The lack of therapeutic interventions in combination with the threat of weaponizing this organism has enhanced research investigations. The expression of key viral proteins and the production of ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Melito PL,Qiu X,Fernando LM,deVarennes SL,Beniac DR,Booth TF,Jones SM

    更新日期:2008-03-01 00:00:00

  • Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

    abstract::Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Shen H,Fang SG,Chen B,Chen G,Tay FP,Liu DX

    更新日期:2009-09-01 00:00:00

  • A reverse-transcription competitive PCR assay based on chemiluminescence hybridization for detection and quantification of hepatitis C virus RNA.

    abstract::A reverse-transcription competitive PCR (RT-cPCR) combined with chemiluminescence hybridization was designed for the detection and quantitative determination of serum hepatitis C virus (HCV) RNA. The concentration of HCV RNA was calculated based on an external standard curve that was generated by coamplification of in...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Young KC,Chang TT,Hsiao WC,Cheng PN,Chen SH,Jen CM

    更新日期:2002-05-01 00:00:00

  • Using RT-PCR and bDNA assays to measure non-clade B HIV-1 subtype RNA.

    abstract::The performance of the new version of RT-PCR assay (Amplicor HIV-1 Monitor v1.5) was assessed. The quantification of non-B subtype HIV-1 plasma RNA (30A, 1C, 1D, 3E, 2F, 3G) obtained using Monitor v1.5 was compared to the former version of this assay (Monitor v1.0) and to the Quantiplex v2.0 bDNA assay. The new primer...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pasquier C,Sandres K,Salama G,Puel J,Izopet J

    更新日期:1999-08-01 00:00:00

  • Use of the polymerase chain reaction for the sensitive detection of St. Louis encephalitis viral RNA.

    abstract::Polymerase chain reaction (PCR) assays were developed for the detection of RNA from the St. Louis encephalitis (SLE) virus. Using computer-assisted analysis of the MSI-7 strain SLE virus genome, two primer pairs were selected from the capsid-coding and the membrane associated protein-coding genes, and one from the env...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


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    更新日期:1992-01-01 00:00:00

  • Indirect measurement of Epstein-Barr virus neutralising antibodies by ELISA.

    abstract::A rapid and effective ELISA for measuring Epstein-Barr virus (EBV)-neutralizing antibodies in human sera was devised to replace the existing cumbersome method involving the inhibition of fetal cord blood B-cell transformation by the virus. The new method will be invaluable for assessing antibody responses in human sub...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Wilson AD,Morgan AJ

    更新日期:1998-07-01 00:00:00

  • Multiplex polymerase chain reaction for human herpesvirus-6, human cytomegalovirus, and human beta-globin DNA.

    abstract::Human cytomegalovirus and human herpesvirus-6 are closely related viruses which cause similar diseases, have similar cellular repositories of latent infection, and may be detected largely in the same types of clinical specimens. DNA amplification appears likely to play an increasing role in the diagnosis of recent and...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: McElhinney LM,Cooper RJ,Morris DJ

    更新日期:1995-06-01 00:00:00

  • Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy.

    abstract::Immunosorbent electron microscopy was used to quantify recombinant baculovirus-generated bluetongue virus (BTV) core-like particles (CLP) in either purified preparations or lysates of recombinant baculovirus-infected cells. The capture antibody was an anti-BTV VP7 monoclonal antibody. The CLP concentration in purified...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Zheng YZ,Hyatt A,Wang LF,Eaton BT,Greenfield PF,Reid S

    更新日期:1999-06-01 00:00:00

  • Characterisation of visna virus reverse transcriptase: a micro scale reverse transcriptase assay adapted for use with an automated cell harvester.

    abstract::The reverse transcriptase of the sheep lentivirus visna/maedi virus has been characterised. Optima for magnesium ion concentration (5-10 mM), potassium ion concentration (150 mM) and pH (8.25) for this enzyme are very similar to those previously described for the human immunodeficiency viruses. The assay used for this...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Sargan DR,Watt NJ,McConnell I

    更新日期:1992-02-01 00:00:00

  • Nonradioactive, photobiotin-labelled DNA probes for the routine diagnosis of barley yellow dwarf virus.

    abstract::Photobiotin was used to prepare biotinylated, nonradioactive nucleic acid probes for the detection of the RNA of barley yellow dwarf virus (BYDV) in plant extracts. A 1.7-kb cDNA of the PAV isolate of BYDV in the plasmid pUC8 vector was biotinylated and then used intact or as sonicated double-stranded DNA fragments. S...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Habili N,McInnes JL,Symons RH

    更新日期:1987-06-01 00:00:00

  • A multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of multiple viruses in citrus trees.

    abstract::A multiplex polymerase chain reaction (mPCR) assay was developed to detect six RNA and one DNA citrus virus: Citrus leaf rugose virus (CLRV), Citrus psorosis virus (CPsV), Citrus tatter leaf virus (CTLV), Citrus tristeza virus (CTV), Citrus variegation virus (CVV), Citrus yellow mosaic virus (CYMV), and Indian citrus ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Roy A,Fayad A,Barthe G,Brlansky RH

    更新日期:2005-10-01 00:00:00

  • Serological diagnosis of influenza A and B infections by enzyme immunoassay. Comparison with the complement fixation test.

    abstract::Paired sera from 784 patients with symptoms of acute respiratory disease were examined for antibodies against influenza A, B and parainfluenza (1 and 3) viruses by complement fixation (CF) and enzyme immunoassay (EIA). The internal variation of the EIA test results was low and an increase of 0.250 in absorbance values...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Julkunen I,Kleemola M,Hovi T

    更新日期:1984-08-01 00:00:00

  • Specificity of two HIV screening tests detecting simultaneously HIV-1 p24 antigen and antibodies to HIV-1 and -2.

    abstract::This study aimed at assessing the specificity of the Elecsys® HIV combi PT in comparison to the ARCHITECT® HIV Ag/Ab Combo. With both of these assays, 3997 unselected sera from patients of a tertiary health care centre in Basel, Switzerland, were screened for HIV. Reactive sera were reanalysed on the VIDAS® HIV Duo Ul...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Blaich A,Buser A,Stöckle M,Gehringer C,Hirsch HH,Battegay M,Klimkait T,Frei R

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  • Development of a generic real-time PCR assay for simultaneous detection of proviral DNA of simian Betaretrovirus serotypes 1, 2, 3, 4 and 5 and secondary uniplex assays for specific serotype identification.

    abstract::Simian betaretroviruses (formerly Type D retroviruses; SRV) are a group of closely related retroviruses for which the natural host species are Asian monkeys of the genus Macaca. Five serotypes have been identified by classical neutralization assays and three additional untyped variants have been reported (SRV(Tsukuba)...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: White JA,Todd PA,Rosenthal AN,Yee JL,Grant R,Lerche NW

    更新日期:2009-12-01 00:00:00

  • Easy-to-use rapid gene amplification method for direct detection of RNA and DNA viruses in sera and feces from various animals.

    abstract::The development of rapid and simple gene amplification tests is required for detection of pathogens to prevent transmission of infectious diseases between animals or from animals to humans. An easy-to-use rapid gene amplification method that can directly detect RNA and DNA viruses in clinical samples was developed. Th...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Segawa T,Kobayashi Y,Sase Y,Itou T,Suzuki M,Endoh T,Nakanishi T,Sakai T

    更新日期:2014-06-01 00:00:00

  • Non-ionic detergents facilitate non-specific binding of M13 bacteriophage to polystyrene surfaces.

    abstract::Phage-displayed random peptide libraries are widely used for identifying peptide interactions with proteins and other substrates. Selection of peptide ligands involves iterative rounds of affinity enrichment. The binding properties of the selected phage clones are routinely tested using immunoassay after propagation t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Hakami AR,Ball JK,Tarr AW

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  • A rapid RT-LAMP assay for the detection of all four lineages of Peste des Petits Ruminants Virus.

    abstract::Peste des petits ruminants (PPR) is a viral disease of small ruminants that is caused by the PPR virus (PPRV) and is a significant burden on subsistence farmers across the developing world. Loop-mediated isothermal amplification (LAMP) provides cost-effective, rapid, specific and sensitive detection of nucleic acid an...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Rajko-Nenow P,Flannery J,Arnold H,Howson ELA,Darpel K,Stedman A,Corla A,Batten C

    更新日期:2019-12-01 00:00:00

  • Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.

    abstract::TaqMan and SYBR Green quantitative PCR (qPCR) methods were developed as DNA-based approaches to reproducibly enumerate M13 and T7 phages from phage display selection experiments individually and simultaneously. The genome copies of M13 and T7 phages were quantified by TaqMan or SYBR Green qPCR referenced against M13 a...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Peng X,Nguyen A,Ghosh D

    更新日期:2018-02-01 00:00:00

  • Evaluation of the Pan Influenza detection kit utilizing the PLEX-ID and influenza samples from the 2011 respiratory season.

    abstract::A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N=75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa=0.57 95% CI 0.3-0.89). Positive percent agreement was 92.3% (60...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: A Murillo L,Hardick J,Jeng K,Gaydos CA

    更新日期:2013-10-01 00:00:00

  • Characterization of molecular clones of porcine endogenous retrovirus-A containing different numbers of U3 repeat boxes in the long terminal repeat region.

    abstract::When full-length molecular clones of porcine endogenous retrovirus (PERV)-A and porcine endogenous retrovirus (PERV)-B were passaged on human cells, an increase in the length of the long terminal repeat (LTR) was reported. A 39-bp repeat box in the LTR U3 region was multimerized dynamically upon replication, acting as...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lee YJ,Park SH,Bae EH,Jung YT

    更新日期:2012-04-01 00:00:00

  • Chemical inactivation of hepatitis B virus: the effect of disinfectants on virus-associated DNA polymerase activity, morphology and infectivity.

    abstract::The inactivation of hepatitis B virus (HBV) using two commercially available disinfectants was analysed. Indirect evidence of virus inactivation was obtained by examining the decrease in HBV-associated DNA polymerase and HBcAg activities after treatment with increasing concentrations of disinfectant. Inactivation was ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Howard CR,Dixon J,Young P,van Eerd P,Schellekens H

    更新日期:1983-09-01 00:00:00

  • Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs.

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    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Zhou L,Sun Y,Wu JL,Mai KJ,Chen GH,Wu ZX,Bai Y,Li D,Zhou ZH,Cheng J,Wu RT,Zhang XB,Ma JY

    更新日期:2018-05-01 00:00:00