当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Immunocapture and direct binding loop mediated isothermal amplification simplify molecular diagnosis of Cyprinid herpesvirus-3.

Immunocapture and direct binding loop mediated isothermal amplification simplify molecular diagnosis of Cyprinid herpesvirus-3.

Abstract：

:Loop mediated isothermal amplification (LAMP) assay is used for rapid diagnosis of Cyprinid herpesvirus-3, formerly designated koi herpesvirus (KHV), with comparable sensitivity to PCR. To reduce the time required for the LAMP assay, an immunocapture (IC) and direct binding (DB) techniques were developed to exclude the DNA extraction step in molecular diagnostic procedures of the virus. Both techniques were evaluated by using PCR and CyHV-3-LAMP assays. The DB-LAMP/PCR assays were more sensitive (detecting 0.1 virus particles/ml) than the IC-LAMP/PCR assays (detecting 1 virus particle/ml). By using the SYBR Green I stain and the DB/LAMP assay the complete CyHV-3 diagnostic process can be achieved within 90 min compared to more than 5 h for the routine PCR assay. Both assays (IC/DB) could amplify successfully CyHV-3 from clinical samples which prove its application to diagnostic tests. The DB-LAMP assay is a simple, rapid, sensitive technique and applicable to the diagnosis of CyHV-3 in the field.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Soliman H,El-Matbouli M

doi

10.1016/j.jviromet.2009.07.021

subject

Has Abstract

pub_date

2009-12-01 00:00:00

pages

91-5

issue

1-2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(09)00347-4

journal_volume

162

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • Rapid method for the identification and screening of herpesviruses by DNA fingerprinting combined with blot hybridization.

    abstract：:Rapid characterization of herpesvirus isolates exemplified by equine herpesviruses is described. Total DNAs were isolated from virus infected small scale cell cultures. The DNA fragments obtained after restriction enzyme digestion were separated on agarose gels, transferred and immobilized on filter membranes. A radio...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(86)90030-3

    authors： Chowdhury SI,Hammerschmidt W,Ludwig H,Thein P,Buhk HJ

    更新日期：1986-11-01 00:00:00

  • Proteasome inhibition reduces avian reovirus replication and apoptosis induction in cultured cells.

    abstract：:The interplay between avian reovirus (ARV) replication and apoptosis and proteasome pathway was studied in cultured cells. It is shown that inhibition of the proteasome did not affect viral entry and host cell translation but had influence on ARV replication and ARV-induced apoptosis. Evidence is provided to demonstra...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.03.016

    authors： Chen YT,Lin CH,Ji WT,Li SK,Liu HJ

    更新日期：2008-07-01 00:00:00

  • Comparative study of peroxidase-labelled IgG and anti-IgG for the detection of measles virus antigens.

    abstract：:The immunoperoxidase and immunoblotting techniques were used to detect measles virus antigens in nasopharyngeal samples taken from children visiting the Yaounde Health Centre (Cameroon). In order to make these diagnostic tests simpler, faster and less costly, peroxidase-labelled anti-measles IgG were prepared and comp...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(90)90011-4

    authors： Njayou M,Balla A

    更新日期：1990-07-01 00:00:00

  • A simple and rapid method of high quantity DNA isolation from cervical scrapes for detection of human papillomavirus infection.

    abstract：:Infection with human papillomavirus (HPV) is an important etiological factor in the development of cervical cancer, and detection of the viral genome is of prognostic importance, particularly for preneoplastic lesions. We developed a simple, easy and efficient non-organic method of DNA extraction from cervical scrapes...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(92)90157-9

    authors： Gopalkrishna V,Francis A,Sharma JK,Das BC

    更新日期：1992-01-01 00:00:00

  • A newly developed immunofluorescence assay for simultaneous detection of antibodies to human immunodeficiency virus type 1 and type 2.

    abstract：:Immunofluorescence assays (IFA) that simultaneously distinguish between antibodies against closely related human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) infections have not been readily available. Serum specimens from 95 HIV-1-infected, 26 HIV-2-infected and 3 HIV-1/HIV-2 dually infected individuals a...

    journal_title：Journal of virological methods

    pub_type： 临床试验,杂志文章

    doi：10.1016/0166-0934(94)00107-r

    authors： Tanabe-Tochikura A,Ang Singh MT,Tsuchie H,Zhang J,Paladin FJ,Kurimura T

    更新日期：1995-04-01 00:00:00

  • Rapid bioassay of human interferon by direct enzyme immunoassay of encephalomyocarditis virus in HEp-2 cell monolayers after a single cycle of infection.

    abstract：:Multiplication of encephalomyocarditis virus (EMCV) in human HEp-2 cells, and its suppression by interferon (IFN), was demonstrated by direct enzyme immunoassay (EIA) in cell culture. EMCV was detected in glutaraldehyde fixed HEp-2 cell monolayers, in wells of 96-well plates, with a horse radish peroxidase (HRPO) labe...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(89)90017-7

    authors： Vlaspolder F,Donkers E,Harmsen T,Kraaijeveld CA,Snippe H

    更新日期：1989-04-01 00:00:00

  • Chicken single-chain variable fragments against the SARS-CoV spike protein.

    abstract：:The major concern for severe acute respiratory syndrome (SARS), caused by the SARS-associated coronavirus (SARS-CoV), is the lack of diagnostic and therapeutic agents. Using a phage display technology in a chicken system, high-affinity monoclonal antibody fragments against the SARS-CoV spike protein were characterized...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.06.010

    authors： Lee YC,Leu SJ,Hu CJ,Shih NY,Huang IJ,Wu HH,Hsieh WS,Chiang BL,Chiu WT,Yang YY

    更新日期：2007-12-01 00:00:00

  • A rapid and sensitive assay for detection of replication-competent adenoviruses by a combination of microcarrier cell culture and quantitative PCR.

    abstract：:The development of a rapid and sensitive assay for detection of replication-competent adenoviruses (RCAs) is described. This RCA assay consists of an incubation step of 4 days of adenoviral vectors on A549 cells in a microcarrier cell culture system followed by detection of amplified RCAs by E1-specific quantitative P...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.05.010

    authors： Schalk JA,de Vries CG,Orzechowski TJ,Rots MG

    更新日期：2007-11-01 00:00:00

  • Development of a highly sensitive real-time RT-PCR protocol for the detection of Classical swine fever virus independent of the 5' untranslated region.

    abstract：:Classical swine fever (CSF) is one of the most severe diseases of pigs, and can cause immense economic losses. Real-time reverse transcription polymerase chain reaction (rRT-PCR) can be used as a sensitive and specific method for detection of Classical swine fever virus (CSFV). Different published protocols are used r...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.11.014

    authors： Leifer I,Blome S,Beer M,Hoffmann B

    更新日期：2011-01-01 00:00:00

  • Highly sensitive and specific non-radioactive biotinylated probes for dot-blot, Southern and colony hybridizations.

    abstract：:We describe a simple method for the incorporation of biotin into nucleic acid probes. This method has been improved and optimized to produce biotinylated DNA probes for the detection of DNA by dot-blot, Southern and colony hybridization techniques. The sensitivity of this method has been particularly improved to allow...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(89)90072-4

    authors： Eweida M,Sit TL,Sira S,AbouHaidar MG

    更新日期：1989-10-01 00:00:00

  • Cloning and expression of two fragments of the S gene of canine coronavirus type I.

    abstract：:Two fragments, S66 and S55, of the S glycoprotein of the newly identified canine coronavirus type I (CCoV type I), were expressed in a procariotic system. The purified recombinant proteins of 350 and 366 amino acids in length, respectively, were employed to develop an enzyme-linked immunosorbent assay (ELISA) for the ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2003.12.003

    authors： Pratelli A,Elia G,Decaro N,Tola S,Tinelli A,Martella V,Rocca S,Tempesta M,Buonavoglia C

    更新日期：2004-04-01 00:00:00

  • Nucleotide-specific PCR for molecular virus typing.

    abstract：:Nucleotide sequence studies detected a double-point mutation in the genomic RNA segment 4 (nt 871 and 872) of the persistent variant C/AA-pi of influenza C/Ann Arbor/1/50 virus. The 3'-end-points of two distinct PCR primers were positioned exactly at this genome location and thereby adjusted the priming determinant co...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(94)00159-e

    authors： Marschall M,Schuler A,Böswald C,Helten A,Hechtfischer A,Lapatschek M,Meier-Ewert H

    更新日期：1995-03-01 00:00:00

  • Development of a RT-nested PCR test detecting pigeon Paramyxovirus-1 directly from organs of infected animals.

    abstract：:A RT-nested PCR that amplifies part of the conserved nucleoprotein gene of avian Paramyxovirus type 1 is described. The technique allowed the detection of pigeon Paramyxovirus type 1 (pPMV-1) virus directly from a wide range of infected chicken and pigeon organs, and should be able to detect typical Newcastle disease ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00148-9

    authors： Barbezange C,Jestin V

    更新日期：2002-12-01 00:00:00

  • Simultaneous detection of enterovirus 70 and coxsackievirus A24 variant by multiplex real-time RT-PCR using an internal control.

    abstract：:Epidemics of acute hemorrhagic conjunctivitis are always explosive and extensive, and have been recognized as a serious international public health problem. Enterovirus 70 and coxsackievirus A24 variant have been identified as the major etiological agents in acute hemorrhagic conjunctivitis outbreaks worldwide. A nove...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.02.022

    authors： Xiao XL,Wu H,Li YJ,Li HF,He YQ,Chen G,Zhang JW,Yang H,Li XF,Yang XQ,Yu YG

    更新日期：2009-07-01 00:00:00

  • HSV-1 amplicon peptide display vector.

    abstract：:There are significant uses for expressing foreign peptide epitopes in viral surface attachment proteins in terms of investigating viral targeting, biology, and immunology. HSV-1 attachment, followed by fusion and entry, is mediated in large part by the binding of viral surface glycoproteins to cell surface receptors, ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00193-3

    authors： Spear MA,Schuback D,Miyata K,Grandi P,Sun F,Yoo L,Nguyen A,Brandt CR,Breakefield XO

    更新日期：2003-01-01 00:00:00

  • Evaluation of cell viability dyes in antiviral assays with RNA viruses that exhibit different cytopathogenic properties.

    abstract：:Studies were conducted to determine the performance of four dyes in assessing antiviral activities of compounds against three RNA viruses with differing cytopathogenic properties. Dyes included alamarBlue® measured by absorbance (ALB-A) and fluorescence (ALB-F), neutral red (NR), Viral ToxGlo™ (VTG), and WST-1. Viruse...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2017.03.012

    authors： Smee DF,Hurst BL,Evans WJ,Clyde N,Wright S,Peterson C,Jung KH,Day CW

    更新日期：2017-08-01 00:00:00

  • A loop-mediated isothermal amplification coupling with a lateral flow dipstick for rapid and specific detection of fowl adenovirus serotype-4.

    abstract：:Fowl adenovirus serotype-4 (FAdV-4) has been recognized as a predominant threat to the broilers aged from three to five weeks. Hydropericardium syndrome (HPS) is one of its major clinical diseases by FAdV-4 resulting in heavy economic losses. In this study, a loop-mediated isothermal amplification coupling with a late...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2019.04.026

    authors： Zhai X,Mei X,Wu X,Zuo L,Zhou L,Tian Y,Han X,Yang X,Wang H

    更新日期：2019-08-01 00:00:00

  • Detection of human herpesviruses HHV-6, HHV-7 and HHV-8 in whole blood by real-time PCR using the new CMV, HHV-6, 7, 8 R-gene kit.

    abstract：:Human herpesviruses 6 (HHV-6), 7 (HHV-7) and 8 (HHV-8) are lymphotropic herpesviruses that may cause opportunistic diseases in immunosuppressed patients such as transplant or AIDS patients. The new commercial CMV HHV-6, 7, 8 R-gene kit (Argene, Varilhes, France) for the simultaneous quantitation of HHV-6 and qualitati...

    journal_title：Journal of virological methods

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.jviromet.2008.01.026

    authors： Deback C,Agbalika F,Scieux C,Marcelin AG,Gautheret-Dejean A,Cherot J,Hermet L,Roger O,Agut H

    更新日期：2008-05-01 00:00:00

  • Duplex SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus and canine astrovirus.

    abstract：:A duplex SYBR Green I-based real-time PCR assay was established for the simultaneous detection of canine kobuvirus (CaKoV) and canine astrovirus (CaAstV). This assay can easily distinguish the two viruses according to their different melting temperatures (Tm) of 80 °C for CaKoV and 86.5 °C for CaAstV; other canine ent...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2021.114066

    authors： Wang Y,Li Y,Cui Y,Jiang S,Liu H,Wang J,Li Y

    更新日期：2021-01-13 00:00:00

  • Evaluation of the automatic editing tool RECall for HIV-1 pol and V3 loop sequences.

    abstract：:Genotypic drug resistance testing is routine practice in HIV-1 clinical care. The visual interpretation of sequencing electropherograms is labour-intensive and subject to intra- and inter-assay variability because decisions are based on operators' judgments. In this study the performance of the automatic editing tool ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.05.017

    authors： Vinken L,Megens S,Schrooten Y,Vandamme AM,Van Laethem K

    更新日期：2013-10-01 00:00:00

  • HPV antibody detection by ELISA with capsid protein L1 fused to glutathione S-transferase.

    abstract：:An alternative enzyme linked immunosorbent assay (ELISA) system was developed to analyze antibodies to human papillomavirus capsid antigens. The assay uses glutathione crosslinked to casein to capture the major capsid protein L1 from human papillomavirus (HPV) types 6b, 16 and 18 fused to glutathione S-transferase (GS...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00134-9

    authors： Sehr P,Müller M,Höpfl R,Widschwendter A,Pawlita M

    更新日期：2002-10-01 00:00:00

  • Simple diffusion-constrained immunoassay for p24 protein with the sensitivity of nucleic acid amplification for detecting acute HIV infection.

    abstract：:Nucleic acid amplification techniques have become the mainstay for ultimate sensitivity for detecting low levels of virus, including human immunodeficiency virus (HIV). As a sophisticated technology with relative expensive reagents and instrumentation, adoption of nucleic acid testing (NAT) can be cost inhibited in se...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.08.017

    authors： Chang L,Song L,Fournier DR,Kan CW,Patel PP,Ferrell EP,Pink BA,Minnehan KA,Hanlon DW,Duffy DC,Wilson DH

    更新日期：2013-03-01 00:00:00

  • Rapid subtyping of H9N2 influenza virus by a triple reverse transcription polymerase chain reaction.

    abstract：:The aim of this study was to develop a rapid, cost-saving triple reverse transcription polymerase chain reaction (triple RT-PCR) for subtyping H9N2 avian influenza viruses (AIVs). The three primer pairs for amplification of target sequences of nucleoprotein (NP), hemagglutinin (HA) and neuraminidase (NA) genes, respec...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.01.026

    authors： Chen HT,Zhang J,Ma LN,Ma YP,Ding YZ,Wang M,Liu XT,Zhang YG,Liu YS

    更新日期：2009-06-01 00:00:00

  • A new system to measure and compare hepatitis C virus replication capacity using full-length, replication competent viruses.

    abstract：:Measuring the in vitro replication capacity of viruses is an important tool for assessing the effects of selective pressure of immune responses and drug therapy. Measuring hepatitis C virus (HCV) replication capacity utilizing primarily sub-genomic reporter constructs is limited. To overcome some of these limitations ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.08.009

    authors： Lassmann B,Arumugaswami V,Chew KW,Lewis MJ

    更新日期：2013-12-01 00:00:00

  • Viral elution and concentration method for detection of influenza A viruses in mud by real-time RT-PCR.

    abstract：:The role of environmental reservoirs in avian influenza virus (AIV) transmission has been investigated during AIV-associated outbreaks. To date, no method has been defined for detection of AIV from mud samples. A procedure using elution and polyethylene glycol (PEG) concentration steps was designed to detect AIV by RT...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2011.10.013

    authors： Deboosere N,Horm SV,Delobel A,Gachet J,Buchy P,Vialette M

    更新日期：2012-01-01 00:00:00

  • Development of a real-time reverse transcription polymerase chain reaction assay for detection of marine caliciviruses (genus Vesivirus).

    abstract：:Marine caliciviruses form a distinct lineage within the genus Vesivirus (family Caliciviridae). This group includes vesicular exanthema of swine virus (VESV) and San Miguel sea lion virus (SMSV) and other related viruses which have been proposed to be marine in origin isolated from a variety of terrestrial and marine ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2006.11.010

    authors： Reid SM,King DP,Shaw AE,Knowles NJ,Hutchings GH,Cooper EJ,Smith AW,Ferris NP

    更新日期：2007-03-01 00:00:00

  • Enzyme-linked immunosorbent assay for IgG and IgM antibodies against human parvovirus B19: use of monoclonal antibodies and viral antigen propagated in vitro.

    abstract：:Enzyme-linked immunosorbent assay (ELISA) systems for serum IgG and IgM antibodies to human parvovirus B19 were established by utilizing anti-B19 monoclonal antibodies (mAbs) and human plasma B19 antigen. The specificities of IgG and IgM ELISA were confirmed by indirect immunofluorescence staining and Western blot imm...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(89)90146-8

    authors： Yaegashi N,Shiraishi H,Tada K,Yajima A,Sugamura K

    更新日期：1989-11-01 00:00:00

  • Evaluation of solubilized herpes simplex virus membrane antigens in diffusion in gel-enzyme-linked immunosorbent assay (DIG-ELISA).

    abstract：:Cell membranes were prepared from herpes simplex virus (HSV) type 1-infected cells and solubilized with a low concentration of sodium deoxycholate. The supernatant after ultracentrifugation was used as antigen in a newly developed solid phase assay, diffusion in gel-enzyme-linked immunosorbent assay (DIG-ELISA). Antig...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(82)90045-3

    authors： Jeansson S,Elwing H,Nygren H,Olofsson S

    更新日期：1982-04-01 00:00:00

  • Serodiagnosis of grass carp reovirus infection in grass carp Ctenopharyngodon idella by a novel Western blot technique.

    abstract：:Frequent outbreaks of grass carp hemorrhagic disease, caused by grass carp reovirus (GCRV) infection, pose as serious threats to the production of grass carp Ctenopharyngodon idella. Although various nucleic acids-based diagnostic methods have been shown effective, lack of commercial monoclonal antibody against grass ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2013.08.001

    authors： He Y,Jiang Y,Lu L

    更新日期：2013-12-01 00:00:00

  • Detection and quantitation of group A rotaviruses by competitive and real-time reverse transcription-polymerase chain reaction.

    abstract：:A competitive reverse transcription-polymerase chain reaction (RT-PCR) was developed to detect and to quantitate the RNA of group A rotaviruses. In the assay, a 433 bp fragment is amplified by a one-tube RT-PCR protocol using primers with binding sites located in a highly conserved region of segment 6 of the rotavirus...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(02)00118-0

    authors： Schwarz BA,Bange R,Vahlenkamp TW,Johne R,Müller H

    更新日期：2002-09-01 00:00:00