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Development of a RT-nested PCR test detecting pigeon Paramyxovirus-1 directly from organs of infected animals.

Abstract：

:A RT-nested PCR that amplifies part of the conserved nucleoprotein gene of avian Paramyxovirus type 1 is described. The technique allowed the detection of pigeon Paramyxovirus type 1 (pPMV-1) virus directly from a wide range of infected chicken and pigeon organs, and should be able to detect typical Newcastle disease viruses too. Compared with the reference method, the developed RT-nested PCR was found more sensitive, as it was able to detect virus genome in infected pigeon organs at late stage of infection, when virus isolation failed. Such a molecular technique represents an alternative method of diagnosis for research purposes on pPMV-1 variants, for example to study pathogenesis aspects of the infection or to assess the efficacy of vaccines.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Barbezange C,Jestin V

doi

10.1016/s0166-0934(02)00148-9

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

197-207

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166093402001489

journal_volume

106

pub_type

杂志文章

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