Abstract:
:Frequent outbreaks of grass carp hemorrhagic disease, caused by grass carp reovirus (GCRV) infection, pose as serious threats to the production of grass carp Ctenopharyngodon idella. Although various nucleic acids-based diagnostic methods have been shown effective, lack of commercial monoclonal antibody against grass carp IgM has impeded the development of any reliable immunoassays in detection of GCRV infection. The present study describes the preparation and screening of monoclonal antibodies against the constant region of grass carp IgM protein, and the development of a Western blot (WB) protocol for the specific detection of antibodies against outer capsid VP7 protein of GCRV that serves as antibody-capture antigen in the immunoassay. In comparison to a conventional RT-PCR method, validity of the WB is further demonstrated by testing on clinical fish serum samples collected from a grass carp farm in Jiangxi Province during disease pandemic in 2011. In conclusion, the WB technique established in this study could be employed for specific serodiagnosis of GCRV infection.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
He Y,Jiang Y,Lu Ldoi
10.1016/j.jviromet.2013.08.001subject
Has Abstractpub_date
2013-12-01 00:00:00pages
14-20issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(13)00333-9journal_volume
194pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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