Abstract：
:Peach rosette mosaic disease was first described in the 1940s affecting peach and plum. It was later determined that peach rosette mosaic virus (PRMV) is the causal agent of the disease. PRMV, a member of the genus Nepovirus, infects several perennial crops including stone fruit, grape and blueberry as well as several weed species found in orchards around the world. The molecular characterization of the virus is limited to partial genome sequences making it difficult to develop reliable and sensitive molecular detection tests; the reason that detection is routinely performed using ELISA with antibodies risen against a single virus isolate. Given the potential economic impact of the virus and the modes of transmission which, in addition to nematodes, include seed we studied PRMV in more depth using a modified dsRNA extraction protocol to obtain the virus genome. We determined the full nucleotide sequence and developed a protocol that detects conserved regions present in RNA 1 and RNA 2, making it an excellent alternative to the detection protocols used today.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Ho T,Harris A,Katsiani A,Khadgi A,Schilder A,Tzanetakis IEdoi
10.1016/j.jviromet.2018.01.004subject
Has Abstractpub_date
2018-04-01 00:00:00pages
8-12eissn
0166-0934issn
1879-0984pii
S0166-0934(17)30640-7journal_volume
254pub_type
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