Abstract:
:The suitability of autoclaved tap water for the preparation of ELISA reagents and washing buffer was compared with that of ultrapure water, in a standard indirect ELISA for the detection of antibodies to pseudorabies virus (PRV). The performance of the assay, using autoclaved tap water (AT-ELISA) compared favourably to that of the standard assay, using ultrapure water (UP-ELISA) in detecting anti-PRV antibodies in sequential serum samples from a pig experimentally infected with PRV. While both the UP-ELISA and AT-ELISA proved reliable in detecting anti-PRV antibodies in a coded proficiency serum panel (n = 60), the AT-ELISA detected fewer positive sera than the UP-ELISA in evaluating a limited number (n = 80) of field samples. The results suggest that autoclaved tap water may be substituted for ultrapure water for the preparation of ELISA reagents when or where ultrapure water may not be available.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Afshar A,Zhao X,Heckert RA,Trotter HCdoi
10.1016/0166-0934(94)90110-4subject
Has Abstractpub_date
1994-02-01 00:00:00pages
275-8issue
2eissn
0166-0934issn
1879-0984pii
0166-0934(94)90110-4journal_volume
46pub_type
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