Detection of episomal banana streak badnavirus by IC-PCR.

abstract：:Hepatitis B micelles containing the p25 component of hepatitis B surface antigen (HBsAg) have been produced by Triton X-100 solubilization followed by ultracentrifugation in linear sucrose gradients. The product was found to resemble micelle forms prepared from plasma-derived HBsAg with the surface being composed of d...

journal_title：Journal of virological methods

authors： Howard CR,Young PR,Lee S,Dixon J,Zuckerman AJ,McAleer WJ,Lehman ED

更新日期：1986-08-01 00:00:00

abstract：:Saint Louis encephalitis virus belongs to Flavivirus genus; Flaviviridae family jointly with other medically important flaviviruses including dengue virus and West Nile virus. The biological properties and functions of prM flavivirus protein are under investigation due to its importance in the generation of infectious...

journal_title：Journal of virological methods

authors： Pupo-Antúnez M,Vázquez S,Sosa AL,Caballero Y,Vásquez Y,Morier L,Álvarez M,Guzmán MG

更新日期：2015-06-15 00:00:00

abstract：:A simple procedure is described for reproducible detection of double stranded (ds) RNAs in leafroll infected grapevines. The procedure involves the extraction of tissues by a medium which preferentially yields dsRNA. The RNA is purified by CF11 cellulose chromatography and gel electrophoresis. The dsRNAs varied in siz...

journal_title：Journal of virological methods

authors： Rezaian MA,Krake LR,Cunying Q,Hazzalin CA

更新日期：1991-02-01 00:00:00

abstract：:One of the major factors determining the incidence of Barley yellow dwarf virus (BYDV) on autumn-sown cereals is the viruliferous state of immigrant winged aphids. This variable is assessed routinely using the enzyme-linked immunosorbant assay (ELISA). However, the threshold for virus detection by ELISA can lead to fa...

journal_title：Journal of virological methods

authors： Fabre F,Kervarrec C,Mieuzet L,Riault G,Vialatte A,Jacquot E

更新日期：2003-06-09 00:00:00

abstract：:Beak and feather disease virus (BFDV) threatens a wide range of endangered psittacine birds worldwide. In this study, we assessed a novel PCR assay and genetic screening method using high-resolution melt (HRM) curve analysis for BFDV targeting the capsid (Cap) gene (HRM-Cap) alongside conventional PCR detection as wel...

journal_title：Journal of virological methods

authors： Das S,Sarker S,Ghorashi SA,Forwood JK,Raidal SR

更新日期：2016-11-01 00:00:00

abstract：:The genomic DNA molecule of tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, was amplified from total DNA extracts of TYLCV-infected tomato (Lycopersicon esculentum) by the use of loop-mediated isothermal amplification (LAMP). The procedure was also used to amplify TYLCV DNA from total DNA ex...

journal_title：Journal of virological methods

authors： Fukuta S,Kato S,Yoshida K,Mizukami Y,Ishida A,Ueda J,Kanbe M,Ishimoto Y

更新日期：2003-09-01 00:00:00

abstract：:Four IgG(1kappa) monoclonal antibodies (mAbs) against Influenza A/Chicken/Vietnam/8/2004 (H5N1) virus are described. Three of these showed neutralizing activities against H5N1 strains from clades 1, 2 and 3 using a retroviral pseudotype or live virus microneutralization assay. In the pseudotype assay, the IC(90) neutr...

journal_title：Journal of virological methods

authors： Oh S,Selleck P,Temperton NJ,Chan PK,Capecchi B,Manavis J,Higgins G,Burrell CJ,Kok T

更新日期：2009-05-01 00:00:00

abstract：:The propagation of Taura syndrome virus (TSV) in primary hemocyte culture of Pacific white shrimp (Penaeus vannamei) was investigated. Purified TSV was inoculated into a 24 h old primary hemocyte culture and the development of cytopathic effects was monitored. The cell morphology started changing within 6 h post-inocu...

journal_title：Journal of virological methods

authors： George SK,Kaizer KN,Betz YM,Dhar AK

更新日期：2011-03-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is a major worldwide health problem, causing chronic hepatitis, liver cirrhosis and primary liver cancer (Hepatocellular carcinoma). HCV encodes a precursor polyprotein that is enzymatically cleaved to release the individual viral proteins. The viral non-structural proteins are cleave...

journal_title：Journal of virological methods

authors： Berdichevsky Y,Zemel R,Bachmatov L,Abramovich A,Koren R,Sathiyamoorthy P,Golan-Goldhirsh A,Tur-Kaspa R,Benhar I

更新日期：2003-02-01 00:00:00

abstract：:Four commercial test kits for parvovirus B19 IgM were evaluated by testing 491 sera assembled into 7 panels. The serum panels were designed to assess sensitivity and specificity of the commercial assays and to reflect the various clinical settings in which acute B19 infection forms part of the differential diagnosis. ...

journal_title：Journal of virological methods

authors： Cohen BJ,Bates CM

更新日期：1995-09-01 00:00:00

abstract：:Nine commercial EIAs for measles-specific IgG were compared with haemagglutination inhibition (HI) and plaque reduction neutralization (PRN). A total of 174 sera selected, to give approximately half of the sera without measles antibody by HI, were tested by all EIAs and HI. However, there was sufficient volume of only...

journal_title：Journal of virological methods

authors： Hesketh L,Charlett A,Farrington P,Miller E,Forsey T,Morgan-Capner P

更新日期：1997-06-01 00:00:00

abstract：:A new procedure for the PCR amplification of unknown DNA sequences adjacent to a known sequence is described. The required but not readily available second primer sequence in the unknown DNA sequence is obtained by creating an overhanging restriction site in the unknown sequence to which a double-stranded oligonucleot...

journal_title：Journal of virological methods

authors： Collasius M,Puchta H,Schlenker S,Valet G

更新日期：1991-04-01 00:00:00

abstract：:Foot-and-mouth disease is one of the most economically important virus diseases of livestock. Two important requirements for the control of this disease are rapid laboratory diagnosis and epidemiological investigation. The use of the polymerase chain reaction method (PCR) to amplify specific nucleic acid regions offer...

journal_title：Journal of virological methods

authors： Höfner MC,Carpenter WC,Donaldson AI

更新日期：1993-04-01 00:00:00

abstract：:Systematic solid-phase synthesis of all possible overlapping nonapeptides of the 1381 amino acid sequence of the Epstein-Barr virus major capsid protein (EBV-MCP) was used to identify the position of linear antigen epitopes on this protein as recognised by human polyclonal antisera. Antisera were selected for reactivi...

journal_title：Journal of virological methods

authors： Middeldorp JM,Meloen RH

更新日期：1988-09-01 00:00:00

abstract：:In situ detection of specific cells offers a unique perspective on the spatial interactions between host immune cells and specific viral pathogens or cancers. Most immunohistochemistry techniques require manual cell counting on biopsied and fixed tissue sections. The availability of sophisticated software packages for...

journal_title：Journal of virological methods

authors： Diem K,Magaret A,Klock A,Jin L,Zhu J,Corey L

更新日期：2015-09-15 00:00:00

abstract：:Immunoblotting ('Western blotting') is routinely used for detection of antibodies against HIV in the diagnosis of HIV infection. We describe an improved procedure, which does not require virus purification and is easy to control for 'false-positive' results. The technique also does not produce erroneous results due to...

journal_title：Journal of virological methods

authors： Thorpe R,Brasher MD,Bird CR,Garrett AJ,Jacobs JP,Minor PD,Schild GC

更新日期：1987-05-01 00:00:00

abstract：:An in situ hybridization technique has been optimised for use on paraffin-embedded sections of tissues collected from cattle infected experimentally with foot-and-mouth disease virus type O1BFS. Tissue was collected 5 days after infection by direct contact. In situ hybridization was carried out using an RNA probe corr...

journal_title：Journal of virological methods

authors： Woodbury EL,Ilott MC,Brown CC,Salt JS

更新日期：1995-01-01 00:00:00

abstract：:Human coronavirus NL63 was identified in 2004 in the Netherlands. Due to the high prevalence and world-wide distribution of this pathogen, it is essential to develop a sensitive and specific detection assay suitable for use in a routine diagnostic laboratory. Techniques based on PCR or real-time PCR are laborious and ...

journal_title：Journal of virological methods

authors： Pyrc K,Milewska A,Potempa J

更新日期：2011-07-01 00:00:00

abstract：:Phage-displayed random peptide libraries are widely used for identifying peptide interactions with proteins and other substrates. Selection of peptide ligands involves iterative rounds of affinity enrichment. The binding properties of the selected phage clones are routinely tested using immunoassay after propagation t...

journal_title：Journal of virological methods

authors： Hakami AR,Ball JK,Tarr AW

更新日期：2015-09-01 00:00:00

abstract：:The consumption of bivalve shellfish is a common cause of foodborne outbreaks of viral origin. The evaluation of the sanitary quality of these products, however, is still based on bacterial indicators of fecal contamination (Reg. (EC) No. 2073/2005 and No.1441/2007) even if it is known that they are not reliable indic...

journal_title：Journal of virological methods

authors： Di Pasquale S,Paniconi M,De Medici D,Suffredini E,Croci L

更新日期：2010-01-01 00:00:00

abstract：:Hyperimmune rat antisera prepared against 5 recent antigenic variants of influenza A (H3N2) viruses were studied for haemagglutination inhibiting (HI) antibodies to the homologous and the heterologous viruses. The ratios of homologous to heterologous reactions varied from one animal to another in immunizations with ea...

journal_title：Journal of virological methods

authors： Pyhälä R,Pyhälä L

更新日期：1987-03-01 00:00:00

abstract：:Standard methods for detecting enteroviruses in environmental samples require cell culture, which is time consuming and expensive. The reverse transcription-polymerase chain reaction (RT-PCR) is a rapid, sensitive method for detecting enteroviruses in water. However, environmental samples often contain substances that...

journal_title：Journal of virological methods

authors： Gantzer C,Senouci S,Maul A,Levi Y,Schwartzbrod L

更新日期：1997-05-01 00:00:00

abstract：:An indirect fluorescent antibody test (IFA) was evaluated using commercial mouse anti-measles monoclonal antibody and FITC-labeled goat anti-mouse immunoglobulin. For measles isolation, specimens were inoculated into Rhesus monkey kidney (RMK) cells and, when available, CV-1 cells. 381 specimens were tested by IFA and...

journal_title：Journal of virological methods

authors： Smaron MF,Saxon E,Wood L,McCarthy C,Morello JA

更新日期：1991-06-01 00:00:00

abstract：:Equine infectious anaemia virus (EIAV) was adapted to the Cf2Th cell line, a heterologous malignant line from canine thymus. A persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffusion and immunofluo...

journal_title：Journal of virological methods

authors： Bouillant AM,Nielsen K,Ruckerbauer GM,Samagh BS,Hare WC

更新日期：1986-07-01 00:00:00

abstract：:Studies were performed to identify a pair of primers, specific for the tick-borne encephalitis (TBE) virus complex of the Flaviviridae, with which to develop a rapid and specific identification system based on reverse transcription and the polymerase chain reaction (RT-PCR). The specificity of a putative primer pair w...

journal_title：Journal of virological methods

authors： Whitby JE,Ni H,Whitby HE,Jennings AD,Bradley LM,Lee JM,Lloyd G,Stephenson JR,Barrett AD

更新日期：1993-11-01 00:00:00

abstract：:Droplet Digital PCR (ddPCR) represents a new and alternative platform to conventional quantitative-PCR (qPCR) for the quantitation of DNA templates. However, the proposed improvement in sensitivity and reproducibility offered by ddPCR is not yet fully proven, partly because the delineation between positive and negativ...

journal_title：Journal of virological methods

authors： Jones M,Williams J,Gärtner K,Phillips R,Hurst J,Frater J

更新日期：2014-06-01 00:00:00

abstract：:A polymerase chain reaction (PCR) based on the use of multiple primers enabling the simultaneous detection of HPV-6b, -11, -16 and -18 in a single tube reaction was developed and validated on cervico-vaginal specimens, including tissues embedded in paraffin. This PCR setting proved to be specific and sensitive, allowi...

journal_title：Journal of virological methods

authors： Anceschi MM,Falcinelli C,Pieretti M,Cosmi EV

更新日期：1990-04-01 00:00:00

abstract：:Clinical, pathological, immunological and virological features of simian varicella virus (SVV) infection in primates closely resemble those of varicella zoster virus (VZV) infection in humans. In ganglia infected latently of humans and monkeys, gene 21 of VZV and SVV is transcribed, respectively. We determined the nuc...

journal_title：Journal of virological methods

authors： Mahalingam R,Wellish M,White T,Gilden DH

更新日期：2000-04-01 00:00:00

abstract：:The results of a comparison of three DNA-detection methods for human parvovirus B19 DNA are described. The sensitivity of detection of virus from hybridization assays using 32P-radiolabeled DNA and RNA probes was compared with a method for enzymatically amplifying specific target DNA sequences (polymerase chain reacti...

journal_title：Journal of virological methods

authors： Salimans MM,Holsappel S,van de Rijke FM,Jiwa NM,Raap AK,Weiland HT

更新日期：1989-01-01 00:00:00

abstract：:An indirect enzyme-linked immunosorbent assay termed rnPRRS ELISA using baculovirus-expressed and affinity-purified viral nucleocapsid protein (rNC) antigen was developed for detecting antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) in swine sera. Sera (1395) originating from different E...

journal_title：Journal of virological methods

authors： Denac H,Moser C,Tratschin JD,Hofmann MA

更新日期：1997-05-01 00:00:00