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Optimization of an in situ hybridization technique for the detection of foot-and-mouth disease virus in bovine tissues using the digoxigenin system.

Abstract：

:An in situ hybridization technique has been optimised for use on paraffin-embedded sections of tissues collected from cattle infected experimentally with foot-and-mouth disease virus type O1BFS. Tissue was collected 5 days after infection by direct contact. In situ hybridization was carried out using an RNA probe corresponding to a region of the 3D gene which codes for the RNA polymerase, and labelled with digoxigenin. Consistent, reproducible signal was detected within the epithelial layers of the palatine tonsil, soft palate and pharyngeal tissue studied. This is the first time that a digoxigenin-based system has been used successfully for FMD virus RNA detection with bovine tissue samples.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Woodbury EL,Ilott MC,Brown CC,Salt JS

doi

10.1016/0166-0934(94)00153-8

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

89-93

issue

1

eissn

0166-0934

issn

1879-0984

pii

0166093494001538

journal_volume

51

pub_type

杂志文章

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