Abstract:
:A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Mekuria G,Ramesh SA,Alberts E,Bertozzi T,Wirthensohn M,Collins G,Sedgley Mdoi
10.1016/j.jviromet.2003.08.014subject
Has Abstractpub_date
2003-12-01 00:00:00pages
65-9issue
1eissn
0166-0934issn
1879-0984pii
S0166093403002684journal_volume
114pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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