Abstract:
:The open reading frame coding for the A-type inclusion body protein (ATI) of monkeypox virus (MPV) was identified and sequenced for two strains. Nucleotide sequence comparison revealed 72-95.3% homology with the reported open reading frame sequences of the ATIs of other orthopoxvirus species, such as variola, vaccinia, cowpox, ectromelia, and camelpox viruses. Each MPV strain contained an 8-bp deletion, which caused a frameshift that introduced a premature stop in the open reading frame at base 2091 relative to the ATI open reading frame of cowpox virus strain Brighton. The sequences enabled a primer pair to be designed that flanked the deletion and specifically amplified a 601-bp fragment that identified and differentiated 19 MPV strains examined from five other Old World orthopoxvirus species examined. The specificity was confirmed by cleavage of the 19 MPV strain amplicons with BglII, which produced three subfragments of expected sized, based on the determined MPV sequences.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Neubauer H,Reischl U,Ropp S,Esposito JJ,Wolf H,Meyer Hdoi
10.1016/s0166-0934(98)00099-8subject
Has Abstractpub_date
1998-10-01 00:00:00pages
201-7issue
2eissn
0166-0934issn
1879-0984pii
S0166093498000998journal_volume
74pub_type
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