Abstract：
:A new, versatile baculovirus vector was developed for the generation of recombinants in the yeast Saccharomyces cerevisiae and for the expression of foreign proteins in both insect larvae and in insect cells. This vector is based on Autographa californica multiple nucleocapsid nucleopolyhedrovirus (AcMNPV) and exploits the 10-kDa protein promoter (p10) for the expression of the foreign gene. The p10 locus was used for the insertion of a yeast-selectable marker system (ARS-URA-URA3) and of a gene for screening and titration of recombinants in insect cells (beta-galactosidase). The polyhedron-positive phenotype of this vector is maintained allowing its use in insect larvae, by feeding polyhedra, and in insect cells, by infecting with budded virus. The generation of this baculovirus vector requires a single recombination step in yeast prior to infection of insect cells, but has the advantage over the vector designed previously (Patel et al., A new method for the isolation of recombinant baculovirus, Nucleic Acids Research 20 (1992) 97-104) that these vectors can also be used in insects.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Heldens JG,Kester HA,Zuidema D,Vlak JMdoi
10.1016/s0166-0934(97)00109-2subject
Has Abstractpub_date
1997-10-01 00:00:00pages
57-63issue
1eissn
0166-0934issn
1879-0984pii
S0166093497001092journal_volume
68pub_type
杂志文章abstract：:A microtiter cell-culture assay is described for measuring neutralizing antibody activity directed against the human immunodeficiency virus (HIV). The assay relies upon inhibition of HIV-mediated cell killing of infected MT-4 lymphoid cells. The assay exhibits comparable sensitivity to two other methods used for such ...
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pub_type： 临床试验,杂志文章
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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