Abstract:
:The development and evaluation of an antibody-capture ELISA for the detection of IgM antibodies to Sindbis (SIN) and West Nile (WN) viruses are described. Comparison of the ELISA results with those obtained by indirect immunofluorescence (IIF) antibody tests using both fluorescein and biotinylated anti-human IgM conjugates, showed that the former technique was both more sensitive and specific than the IIF methods. There were no false positives by the ELISA whereas with the IgM-IIF assays a high percentage of false positives were obtained. These were due to rheumatoid factor and also to an interfering factor which was not detected by the RF latex agglutination test. Absorption of the sera with anti-IgG was necessary to eliminate this interference in the IgM-IIF tests.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Besselaar TG,Blackburn NK,Aldridge Ndoi
10.1016/0166-0934(89)90060-8subject
Has Abstractpub_date
1989-09-01 00:00:00pages
337-45issue
3eissn
0166-0934issn
1879-0984pii
0166-0934(89)90060-8journal_volume
25pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
pub_type: 杂志文章
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