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A modified immunofluorescence test for Epstein-Barr virus-specific IgM antibody.

Abstract：

:The fluorescent antibody (FA) test for Epstein-Barr virus (EBV)-specific IgM antibody was improved by the use of sodium butyrate to induce a higher level of EBV antigen expression in P3HR-1 slide preparations and by removal of rheumatoid factor (RF) and IgG antibodies from test sera by means of adsorption with suspensions of Sepharose-IgG and Streptococcus pyogenes strain AR1. This method was compared with the Paul-Bunnell test (PB) on 1106 sera submitted to a routine virus diagnostic laboratory for infectious mononucleosis serology and 96.4% of sera showed concordant results. Thus the EBV-IgM-FA method was suitable for routine diagnostic use. However, it proved helpful to test EBV-IgM positive sera by PB to assist in the detection of cross-reacting IgM antibodies sometimes present.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Harnett GB,Palmer CA,Bucens MR

doi

10.1016/0166-0934(85)90004-7

subject

Has Abstract

pub_date

1985-10-01 00:00:00

pages

25-30

issue

1-2

eissn

0166-0934

issn

1879-0984

pii

0166-0934(85)90004-7

journal_volume

12

pub_type

杂志文章

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