Abstract:
:The humoral immune response of sheep infected with bluetongue virus serotypes 3, 9 and 16 was monitored by plaque inhibition (PI), blocking ELISA (BELISA) and indirect ELISA over a period of 63 days post-infection. Results indicated that testing of a single plasma or serum sample by both a BELISA and an indirect ELISA using a recombinant streptococcal protein G (PrG) peroxidase conjugate enabled an assessment of the proximity of a recent infection based on the failure of PrG to bind ovine IgM class antibodies. When BELISA and indirect ELISA results were expressed as a ratio, values indicative of recent infection (> or = 5) were observed for an average duration of 16.5 days (range 8 to 23 days) following the initial detection of antibody by BELISA. This approach has potential to improve diagnosis of a wide range of virus infections by providing an indicator for the relationship of serological status with a recent infection. However, where reinfection may occur, as with bluetongue virus, alternative methods may be required for definitive diagnosis.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Lunt RA,Blacksell SD,Newberry KMdoi
10.1016/0166-0934(94)90088-4subject
Has Abstractpub_date
1994-06-01 00:00:00pages
53-63issue
1eissn
0166-0934issn
1879-0984pii
0166-0934(94)90088-4journal_volume
48pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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abstract::The mosaic disease caused by fig mosaic virus (FMV) is considered the plague of fig worldwide. A naïve phage display library, raised against the recombinant nucleocapsid protein of FMV (FMV-Np) was screened to obtain specific monoclonal recombinant antibodies in the form of single chain variable fragments (scFvs). Aft...
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pub_type: 杂志文章
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:1982-09-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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journal_title:Journal of virological methods
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更新日期:2008-05-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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journal_title:Journal of virological methods
pub_type: 杂志文章
doi:10.1016/0166-0934(96)02057-5
更新日期:1996-07-01 00:00:00
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
abstract::Development of a HIV-1 vaccine is a major global priority. The yellow fever virus (YFV) attenuated vaccine 17D is among the most effective of currently used vaccines. However, the stability of the YFV17D vector when carrying non-flavivirus genes has been problematic. We have constructed and expressed HIV-1 Env in YFV1...
journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:1984-05-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Journal of virological methods
pub_type: 杂志文章
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