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Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction.

Abstract：

:The genomic DNA molecule of tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, was amplified from total DNA extracts of TYLCV-infected tomato (Lycopersicon esculentum) by the use of loop-mediated isothermal amplification (LAMP). The procedure was also used to amplify TYLCV DNA from total DNA extracts of individual whiteflies (Bemisia tabaci) that had fed on TYLCV-infected plants. One of the characteristics of the LAMP method is its ability to synthesize an extremely large amount of DNA. Accordingly, a large amount of by-product, pyrophosphate ion, is produced yielding a white precipitate of magnesium pyrophosphate in the reaction mixture. The presence or absence of this white precipitate allows easy detection of amplification of TYLCV genomic DNA without gel electrophoresis.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Fukuta S,Kato S,Yoshida K,Mizukami Y,Ishida A,Ueda J,Kanbe M,Ishimoto Y

doi

10.1016/s0166-0934(03)00187-3

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

35-40

issue

1-2

eissn

0166-0934

issn

1879-0984

pii

S0166093403001873

journal_volume

112

pub_type

杂志文章

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