Abstract：
:A simple, sensitive and specific method using the polymerase chain reaction (PCR) for amplification of human immunodeficiency virus type 1 (HIV-1) is described. The method involves minimal manipulations. Peripheral blood mononuclear cells (PBMC) were prepared by a rapid Ficoll-Paque gradient method. Lymphocytes were lysed in PCR buffer containing Proteinase K and detergents, and subjected to amplification under stringent conditions, using two primer pairs. Amplified DNA sequences were hybridized with a 3'-end labelled probe, electrophoresed on agarose gels and visualised by ethidium bromide staining. Identification of amplified HIV-1 proviral DNA sequences was confirmed by autoradiography. HIV-1 sequences were amplified in all samples from 103 HIV-1 seropositive individuals, but not in 40 HIV-1 seronegative controls. The absence of contamination may be attributable in part to minimisation of manipulations before amplification.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Gibson KM,McLean KA,Clewley JPdoi
10.1016/0166-0934(91)90058-8subject
Has Abstractpub_date
1991-05-01 00:00:00pages
277-86issue
2-3eissn
0166-0934issn
1879-0984pii
0166-0934(91)90058-8journal_volume
32pub_type
杂志文章abstract：:Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assa...
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(92)90006-y
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(88)90103-6
更新日期：1988-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0166-0934(99)00134-2
更新日期：2000-01-01 00:00:00
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更新日期：1994-04-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 临床试验,杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2008.11.020
更新日期：2009-04-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2018.02.017
更新日期：2018-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2017.10.011
更新日期：2018-01-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(00)00166-x
更新日期：2000-07-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2012.10.007
更新日期：2013-04-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(96)02004-6
更新日期：1996-04-26 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2013.09.006
更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.jviromet.2005.05.011
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更新日期：2013-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(88)90145-0
更新日期：1988-06-01 00:00:00
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doi：10.1016/j.jviromet.2006.07.023
更新日期：2006-12-01 00:00:00
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doi：10.1016/0166-0934(81)90051-3
更新日期：1981-10-01 00:00:00
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更新日期：2017-06-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2008-12-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/s0166-0934(98)00048-2
更新日期：1998-07-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2010.02.012
更新日期：2010-06-01 00:00:00
abstract：:Poxviruses are emerging pathogens in cetaceans, temporarily named 'Cetaceanpoxvirus' (CePV, family Poxviridae), classified into two main lineages: CePV-1 in odontocetes and CePV-2 in mysticetes. Only a few studies performed the molecular detection of CePVs, based on DNA-polymerase gene and/or DNA-topoisomerase I gene ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2018.06.002
更新日期：2018-09-01 00:00:00
abstract：:A nanoparticle-based immunoassay was developed for the rapid and sensitive detection of avian influenza virus (AIV). In this method, AIV-specific pentabody (pVHH3B) was conjugated to magnetic nanoparticles (MNPs) and used to capture AIV. Gold nanoparticles (GNPs), labelled with the anti-AIV mouse monoclonal antibody 3...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2010.07.024
更新日期：2010-11-01 00:00:00
