A preliminary evaluation of five HIV antigen detection assays.


:Five commercial assays for detecting HIV antigen were evaluated using a panel of 40 coded samples in six laboratories. All assays were capable of detecting HIV-1 antigen (HIV-1 Ag), and are likely to prove useful for monitoring supernatant fluids from a variety of cell cultures. The concentration of HIV-1 protein which the assays detected varied from 25 ng/ml down to 25 pg/ml. Three of the five assays were also able to detect HIV-2 Ag. More extensive evaluations are needed to determine the sensitivity and specificity of these assays on serum samples and body fluids.


J Virol Methods


Healey DS,Maskill WJ,Neate EV,Beaton F,Gust ID




Has Abstract


1988-06-01 00:00:00














  • A novel approach to the detection of classical swine fever virus by RT-PCR with a fluorogenic probe (TaqMan).

    abstract::Detection of classical swine fever virus (CSFV) and its discrimination from other pestiviruses can be achieved by virus isolation (VI) in cell cultures, antigen detection, or molecular analysis. To simplify the latter, a 5'-nuclease assay (TaqMan) was developed for the rapid and specific detection of CSFV with the min...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: McGoldrick A,Lowings JP,Ibata G,Sands JJ,Belak S,Paton DJ

    更新日期:1998-06-01 00:00:00

  • Comparison of two hybridization assays for the rapid detection of PCR amplified HSV genome sequences from cerebrospinal fluid.

    abstract::Rapid diagnosis of herpes simplex encephalitis (HSE) can only be achieved by the polymerase chain reaction (PCR). In order to carry out PCR under routine conditions, it is of great importance to establish an easy DNA extraction protocol and especially a rapid and sensitive DNA detection method. In the present study, t...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Sakrauski A,Weber B,Kessler HH,Pierer K,Doerr HW

    更新日期:1994-12-01 00:00:00

  • Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus.

    abstract::Clinical detection of Middle East respiratory syndrome (MERS) coronavirus (MERS-CoV) in patients is achieved using genetic diagnostic methods, such as real-time RT-PCR assay. Previously, we developed a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of MERS-CoV [Virol J. ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Shirato K,Semba S,El-Kafrawy SA,Hassan AM,Tolah AM,Takayama I,Kageyama T,Notomi T,Kamitani W,Matsuyama S,Azhar EI

    更新日期:2018-08-01 00:00:00

  • Detection of bovine leukemia virus in cattle by the polymerase chain reaction.

    abstract::Bovine leukemia virus (BLV) is widely distributed in U.S. cattle herds. It infects B lymphocytes and causes neoplastic disease in 5-10% of infected animals. Direct economic losses are incurred as a result of death, reduced milk production and condemnation at slaughter. Thus the identification of cattle infected with B...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Murtaugh MP,Lin GF,Haggard DL,Weber AF,Meiske JC

    更新日期:1991-06-01 00:00:00

  • A plasmid-based reverse genetics system for mammalian orthoreoviruses driven by a plasmid-encoded T7 RNA polymerase.

    abstract::Mammalian orthoreoviruses (reoviruses) have served as highly useful models for studies of virus replication and pathogenesis. The development of a plasmid-based reverse genetics system represented a major breakthrough in reovirus research. The current reverse genetics systems for reoviruses rely on the expression of T...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Komoto S,Kawagishi T,Kobayashi T,Ikizler M,Iskarpatyoti J,Dermody TS,Taniguchi K

    更新日期:2014-02-01 00:00:00

  • A near-full length genotypic assay for HCV1b.

    abstract::A near-full genome genotypic assay for HCV1b was developed, which may prove useful to investigate antiviral drug resistance, given new combination therapies for HCV1 infection. The assay consists of three partially overlapping PCRs followed by Sanger population or Illumina next-generation sequencing. Seventy-seven the...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Cuypers L,Snoeck J,Vrancken B,Kerremans L,Vuagniaux G,Verbeeck J,Nevens F,Camacho RJ,Vandamme AM,Van Dooren S

    更新日期:2014-12-01 00:00:00

  • Accuracy of rotavirus diagnosis: modified genome electrophoresis versus electron microscopy.

    abstract::530 human faecal samples were examined for the presence of rotavirus by electron microscopy (EM) and by a modified electrophoretic analysis of viral genome (PAGE). 516 stools gave identical results by both methods (97.4% agreement). The proportion of EM+, PAGE- samples (1/530) was significantly lower than the proporti...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pyndiah N,Béguin R,Richard J,Charles M,Rey A,Bonifas V

    更新日期:1988-05-01 00:00:00

  • Construction, characterization and immunogenicity of a glycoprotein E negative bovine herpesvirus-1.1 Egyptian strain "Abu-Hammad".

    abstract::A full glycoprotein E (gE) deletion was generated in genome of the Egyptian BoHV-1.1 Abu-Hammad strain. Integrity of the gE negative (gE(-)) mutant virus was proved by successful specific PCR amplifications of gB, gC, tk, gD, gI and gE genes along with definite immune reaction to polyclonal anti-BoHV-1 antibody in inf...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: El-Kholy AA,Rady DI,Abdou ER,Elseafy MM,Abdelrahman KA,Soliman H

    更新日期:2013-12-01 00:00:00

  • Development of a real-time reverse transcription polymerase chain reaction assay for detection of marine caliciviruses (genus Vesivirus).

    abstract::Marine caliciviruses form a distinct lineage within the genus Vesivirus (family Caliciviridae). This group includes vesicular exanthema of swine virus (VESV) and San Miguel sea lion virus (SMSV) and other related viruses which have been proposed to be marine in origin isolated from a variety of terrestrial and marine ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Reid SM,King DP,Shaw AE,Knowles NJ,Hutchings GH,Cooper EJ,Smith AW,Ferris NP

    更新日期:2007-03-01 00:00:00

  • Purification and characterization of hepatitis B virus surface antigen particles produced in Drosophila Schneider-2 cells.

    abstract::The small surface antigen of hepatitis B virus (HBV) was produced in Drosophila melanogaster Schneider-2 (DS-2) cells transfected stably using an inducible Drosophila metallothionein promoter. Selected clonal DS-2 cell-lines expressed and secreted large quantities of HBsAg particles consisting exclusively of non-glyco...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Deml L,Schirmbeck R,Reimann J,Wolf H,Wagner R

    更新日期:1999-05-01 00:00:00

  • Development and characterization of mouse monoclonal antibodies against monomeric dengue virus non-structural glycoprotein 1 (NS1).

    abstract::Dengue virus (DENV) nonstructural-1 (NS1) glycoprotein is useful for diagnosis of DENV infections in the first 8 days of illness with any of the four serotypes (DENV-1, DENV-2, DENV-3 and DENV-4). However, NS1 diagnostics are less sensitive for secondary DENV infections so the utility of NS1 diagnostics in dengue ende...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Gelanew T,Poole-Smith BK,Hunsperger E

    更新日期:2015-09-15 00:00:00

  • Real-time quantitative PCR for assessment of antiviral drug effects against Epstein-Barr virus replication and EBV late mRNA expression.

    abstract::This study assesses the ability of quantitative real-time PCR to measure the effects of virus DNA polymerase inhibitors on EBV DNA and late mRNAs syntheses in EBV-producing cell lines. In-house real-time quantitative PCRs were used to measure EBV DNA (thymidine kinase) and mRNAs (BLLF1 gene/gp350/220, BVRF2 gene/prote...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Ballout M,Germi R,Fafi-Kremer S,Guimet J,Barguès G,Seigneurin JM,Morand P

    更新日期:2007-07-01 00:00:00

  • A novel method of HPV genotyping using Hybrid Capture sample preparation method combined with GP5+/6+ PCR and multiplex detection on Luminex XMAP.

    abstract::A novel DNA detection assay comprising Hybrid Capture sample preparation, GP5+/6+ PCR with modifications and Luminex 100 detection was developed and applied to genotyping of human papillomavirus (HPV) in cervical samples. Target-specific sample preparation was performed using magnetic beads conjugated with Hybrid Capt...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Nazarenko I,Kobayashi L,Giles J,Fishman C,Chen G,Lorincz A

    更新日期:2008-12-01 00:00:00

  • Mass spectrometric detection of the amino acid sequence polymorphism of the hepatitis C virus antigen.

    abstract::A method for detection and identification of the hepatitis C virus antigen (HCVcoreAg) in human serum with consideration for possible amino acid substitutions is proposed. The method is based on a combination of biospecific capturing and concentrating of the target protein on the surface of the chip for atomic force m...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kaysheva AL,Ivanov YD,Frantsuzov PA,Krohin NV,Pavlova TI,Uchaikin VF,Konev VА,Kovalev OB,Ziborov VS,Archakov AI

    更新日期:2016-03-01 00:00:00

  • Characterization of isolates of Citrus tristeza virus by sequential analyses of enzyme immunoassays and capillary electrophoresis-single-strand conformation polymorphisms.

    abstract::Citrus tristeza virus (CTV) is the causal agent of tristeza disease, which is one of the most devastating diseases of citrus crops worldwide. This paper describes a method for the rapid detection and genotyping of naturally spreading CTV isolates. This method uses ELISA or dot-blot immunological tests to detect trees ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Licciardello G,Raspagliesi D,Bar-Joseph M,Catara A

    更新日期:2012-05-01 00:00:00

  • Analysis of HCV resistance mutations during combination therapy with protease inhibitor boceprevir and PEG-IFN alpha-2b using TaqMan mismatch amplification mutation assay.

    abstract::TaqMan Mismatch Amplification Mutation Assay (TaqMAMA) is a highly sensitive allelic discrimination method. The mismatch amplification mutation assay (MAMA) is based on preferential amplification of mutant allele by the 'MAMA' primer, which is designed to have two mismatches with the wild-type allele and only one mism...

    journal_title:Journal of virological methods

    pub_type: 杂志文章,随机对照试验


    authors: Curry S,Qiu P,Tong X

    更新日期:2008-11-01 00:00:00

  • Simplified dengue virus microwell plaque assay using an automated quantification program.

    abstract::The plaque assay is essential for virion quantitation but the classic protocol requires considerable efforts. A simplified dengue 96-well plaque assay with automated quantitation program is an alternative to access the level of infectious virus. Dengue plaque assay was simplified using LLC/MK2 cells and virus mixing s...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Boonyasuppayakorn S,Suroengrit A,Srivarangkul P,Yuttithamnon W,Pankaew S,Saelee T,Prompetchara E,Salakij S,Bhattarakosol P

    更新日期:2016-11-01 00:00:00

  • Development and evaluation of a reverse transcription loop-mediated isothermal amplification assay for H3N8 equine influenza virus.

    abstract::Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was applied to the detection of equine influenza virus (EIV). Because equine influenza is caused currently by EIV of the H3H8 subtype, the RT-LAMP primer set was designed to target the hemagglutinin gene of this subtype. The detection limit of the ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Nemoto M,Yamanaka T,Bannai H,Tsujimura K,Kondo T,Matsumura T

    更新日期:2011-12-01 00:00:00

  • Quantification of replication of clinical cytomegalovirus isolates in cultured endothelial cells and fibroblasts by a focus expansion assay.

    abstract::A method for quantitative analysis of the growth properties of human cytomegalovirus (HCMV) in various cell culture systems was developed. Recent HCMV isolates are, in most cases cell associated, causing only limited cytopathic effect. This renders comparative analysis of the biological properties of such isolates dif...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Sinzger C,Knapp J,Plachter B,Schmidt K,Jahn G

    更新日期:1997-01-01 00:00:00

  • Real-time PCR assay to identify variants of Vaccinia virus: implications for the diagnosis of bovine vaccinia in Brazil.

    abstract::Naturally occurring infections of Vaccinia virus (VACV) have been recognized in Brazil during the past 10 years. Human Brazilian Vaccinia virus (BVV) infections typically occur as a zoonosis transferred from affected dairy cows to their handlers. Outbreaks have caused notable economic losses to the rural community in ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: de Souza Trindade G,Li Y,Olson VA,Emerson G,Regnery RL,da Fonseca FG,Kroon EG,Damon I

    更新日期:2008-09-01 00:00:00

  • Generation of a porcine alveolar macrophage cell line for the growth of porcine reproductive and respiratory syndrome virus.

    abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) has a marked tropism for cells of the monocyte-macrophage lineage and accordingly, replicates in fully differentiated alveolar macrophages in the natural host. Despite the identification of several putative receptors for PRRSV on porcine alveolar macrophages ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Lee YJ,Park CK,Nam E,Kim SH,Lee OS,Lee du S,Lee C

    更新日期:2010-02-01 00:00:00

  • Monoclonal antibody against Saint Louis encephalitis prM viral protein.

    abstract::Saint Louis encephalitis virus belongs to Flavivirus genus; Flaviviridae family jointly with other medically important flaviviruses including dengue virus and West Nile virus. The biological properties and functions of prM flavivirus protein are under investigation due to its importance in the generation of infectious...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Pupo-Antúnez M,Vázquez S,Sosa AL,Caballero Y,Vásquez Y,Morier L,Álvarez M,Guzmán MG

    更新日期:2015-06-15 00:00:00

  • Sensitive detection of nucleic acids and protein of human papillomavirus type 6 in respiratory and genital tract papillomata.

    abstract::We have developed a sensitive method to detect and localize HPV-6 viral DNA, mRNA and protein in biopsy specimens of genital and respiratory tract lesions by using in situ hybridization and immunoperoxidase assays on sections of plastic-embedded tissue. This modified in situ hybridization technique, using ultrathin se...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Wu TC,Mounts P

    更新日期:1989-07-01 00:00:00

  • Development of an enzyme immunoassay for the Hawaii agent of viral gastroenteritis.

    abstract::The Hawaii agent is a Norwalk-like virus of acute gastroenteritis in humans which is antigenically distinct from the prototype Norwalk agent. We established a solid phase sandwich type microtiter enzyme immunoassay (EIA) for Hawaii antigen employing sera and stools from experimentally challenged volunteers as reagents...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Treanor JJ,Madore HP,Dolin R

    更新日期:1988-12-01 00:00:00

  • Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

    abstract::Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Shen H,Fang SG,Chen B,Chen G,Tay FP,Liu DX

    更新日期:2009-09-01 00:00:00

  • Comparison of multiple viral population characterization methods on a candidate cross-protection Citrus tristeza virus (CTV) source.

    abstract::Citrus tristeza virus (CTV) is the most economically important virus found on citrus and influences production worldwide. The 3' half of the RNA genome is generally conserved amongst sources, whereas the 5' portion is more divergent, allowing for the classification of the virus into a number of genotypes based on sequ...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Kleynhans J,Pietersen G

    更新日期:2016-11-01 00:00:00

  • Comparative study of peroxidase-labelled IgG and anti-IgG for the detection of measles virus antigens.

    abstract::The immunoperoxidase and immunoblotting techniques were used to detect measles virus antigens in nasopharyngeal samples taken from children visiting the Yaounde Health Centre (Cameroon). In order to make these diagnostic tests simpler, faster and less costly, peroxidase-labelled anti-measles IgG were prepared and comp...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Njayou M,Balla A

    更新日期:1990-07-01 00:00:00

  • Observations on polymerase chain reaction amplification of infectious bursal disease virus dsRNA.

    abstract::Two methods for denaturing double stranded (ds)RNA of infectious bursal disease virus for the purpose of reverse transcribing it were compared: Heat denaturation at 65 degrees C in the presence of DMSO and in the absence of DMSO. As part of the analysis, the nature of cDNA in the two preparations was examined by polym...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Qian B,Kibenge FS

    更新日期:1994-04-01 00:00:00

  • Virus isolation and full-length genome sequencing of a representative canine distemper virus wild type strain of the South America 2 clade.

    abstract::Canine Distemper Virus (CDV) is a highly contagious pathogen of dogs that causes severe respiratory, gastrointestinal and nervous signs. Although vaccines have been used to prevent infections, CDV has been reported worldwide, even in vaccinated animals. In the present study, a representative wild type CDV strain (Arg2...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Romanutti C,Keller L,La Torre J,Panzera Y,Fuques E,Pérez R,Gallo Calderon M

    更新日期:2020-05-01 00:00:00

  • A real time RT-PCR assay for the specific detection of Peste des petits ruminants virus.

    abstract::Peste des petits ruminants virus (PPRV) causes a devastating disease of small ruminants present across much of Africa and Asia. Recent surveillance activities and phylogenetic analyses have suggested that the virus is an emerging problem as it is now being detected in areas previously free of the disease. As such, the...

    journal_title:Journal of virological methods

    pub_type: 杂志文章


    authors: Batten CA,Banyard AC,King DP,Henstock MR,Edwards L,Sanders A,Buczkowski H,Oura CC,Barrett T

    更新日期:2011-02-01 00:00:00