Abstract:
:Two methods for denaturing double stranded (ds)RNA of infectious bursal disease virus for the purpose of reverse transcribing it were compared: Heat denaturation at 65 degrees C in the presence of DMSO and in the absence of DMSO. As part of the analysis, the nature of cDNA in the two preparations was examined by polymerase chain reaction (PCR) amplification, firstly by varying the number of cycles of PCR, and secondly by re-amplification of serial dilutions of the reaction products. The results show that denaturation of dsRNA in the presence of DMSO (method 1) is superior to denaturation without DMSO (method 2) judging by the yield of a specific PCR fragment after 30 cycles, and that the products of method 2 can be re-amplified, albeit poorly, with the generation of heterologous products.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Qian B,Kibenge FSdoi
10.1016/0166-0934(94)90081-7subject
Has Abstractpub_date
1994-04-01 00:00:00pages
237-42issue
1-2eissn
0166-0934issn
1879-0984pii
0166-0934(94)90081-7journal_volume
47pub_type
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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journal_title:Journal of virological methods
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更新日期:2013-12-01 00:00:00
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journal_title:Journal of virological methods
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