Abstract：
:Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Schildgen O,Jebbink MF,de Vries M,Pyrc K,Dijkman R,Simon A,Müller A,Kupfer B,van der Hoek Ldoi
10.1016/j.jviromet.2006.07.023subject
Has Abstractpub_date
2006-12-01 00:00:00pages
207-10issue
1-2eissn
0166-0934issn
1879-0984pii
S0166-0934(06)00279-5journal_volume
138pub_type
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