Abstract：
:Rapid detection of novel swine origin influenza A virus (S-OIV) (H1N1) is crucial for timely implementation of infection control measures. In this study, a haemagglutinin (HA) gene-based real-time nucleic acid sequence-based amplification (NASBA) assay was developed for the specific detection of S-OIV (H1N1). The assay was evaluated and validated by comparing it with existing detection methods for S-OIV (H1N1). Results obtained in a 10-fold dilution series assay demonstrated the analytic sensitivity of the present assay was comparable to that of a commercial S-OIV (H1N1) real-time RT-PCR kit and higher than that of the Centers for Disease Control and Prevention (CDC) TaqMan assay. The actual detection limit of the real-time NASBA assay was approximately 50 copies per reaction. Compared with reference methods (viral culture, conventional RT-PCR, and real-time RT-PCR), the sensitivity, specificity, positive predictive value, and negative predictive value of the present assay were all 100%. Overall, the results showed that the real-time NASBA assay could be used for sensitive and specific detection of S-OIV (H1N1).
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Ge Y,Cui L,Qi X,Shan J,Shan Y,Qi Y,Wu B,Wang H,Shi Zdoi
10.1016/j.jviromet.2009.10.025subject
Has Abstractpub_date
2010-02-01 00:00:00pages
495-7issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(09)00464-9journal_volume
163pub_type
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