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Simple and specific method for detection of antibodies against hepatitis E virus in mammalian species.

Abstract：

:Hepatitis E virus (HEV) is the causative agent of hepatitis E, a food- and water-borne disease. In developed countries, consumption of meats from pigs, wild boars and deer is a major source of infection. Although HEV and HEV-related viruses have been detected in many animal species, their zoonotic potential and prevalence has not been completely understood. To detect anti-HEV antibody in mammalian species, a simple enzyme-linked immunosorbent assay (ELISA) was established using extract from cells expressing HEV capsid protein and protein A/G as an antigen and a reagent for detection of antibody. Absorbance in the ELISA was compared with those in our previous ELISA using VLPs and anti-swine antibody, suggesting that newly established ELISA was similarly specific and sensitive as the previous ELISA. Seroprevalence of HEV infection among wild boars was examined in Yamaguchi Prefecture, confirming that 111 of 364 wild boars (30.5%) were positive for anti-HEV antibody. Next, this ELISA was applied to humans, dogs, cats, ferrets, raccoons and masked palm civets in Japan, and anti-HEV antibodies were detected in humans, ferrets, dogs and cats. This ELISA is thus useful for serological surveys and comparison of HEV infection among various mammals, including humans.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Yonemitsu K,Terada Y,Kuwata R,Nguyen D,Shiranaga N,Tono S,Matsukane T,Yokoyama M,Suzuki K,Shimoda H,Takano A,Muto M,Maeda K

doi

10.1016/j.jviromet.2016.07.030

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

56-61

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(16)30158-6

journal_volume

238

pub_type

杂志文章

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