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Expression of Coxsackievirus B4 proteins VP0 and 2C in Escherichia coli and generation of virus protein recognizing antisera.

Abstract：

:Coxsackievirus B4 (CBV-4) capsid protein VP0 and non-structural 2C protein were expressed and purified using a glutathione-S-transferase (GST) fusion protein expression system. We used a full-size CBV-4 cDNA as a template to amplify the genes by polymerase chain reaction (PCR). The genes were cloned into expression vector pGEX-2T and expressed as a fusion protein with GST. The GST-fusion proteins (GST-2C and GST-VP0) were purified in denatured and native forms and used to generate antibodies in rabbits. The antisera raised against GST-VP0 fusion protein recognized the corresponding structural proteins (VP0, VP2 and VP4) from purified CBV-4 preparations and infected cell lysates. In addition, cross-reactivity with CAV-9 and CBV-5 capsid proteins was observed. Anti-GST-2C antisera precipitated viral 2C protein in CBV-4-infected GMK cells, showing that the antibodies recognize the corresponding natural antigen.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Härkönen T,Hovi T,Roivainen M

doi

10.1016/s0166-0934(97)00150-x

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

147-58

issue

1-2

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(97)00150-X

journal_volume

69

pub_type

杂志文章

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