Abstract：
:A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay has been developed to detect and differentiate between canine parvovirus (CPV) and mink enteritis virus (MEV). Eight CPV and three MEV epidemic strains isolated from 28 pathological samples from dogs and minks suspected of being infected with parvovirus were amplified by PCR using a pair of specific primers designed based on the CPV-N strain (M19296). PCR amplified a fragment of 1016bp from the genomic DNA of both MEV and CPV. The MEV-derived fragment could be digested with the restriction enzyme BSP1407I into three fragments of 102bp, 312bp and 602bp, while the fragment amplified from the CPV genomic DNA was digested into only two fragments of 414bp and 602bp. The lowest DNA concentration of CPV and MEV that could be detected using this assay was 0.004μg/ml and 0.03μg/ml, respectively. The PCR-RFLP assay developed in the present study can, therefore, be used to detect and differentiate MEV from CPV with high specificity and sensitivity.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Zhang C,Yu Y,Yang H,Li G,Yu Z,Zhang H,Shan Hdoi
10.1016/j.jviromet.2014.09.014subject
Has Abstractpub_date
2014-12-15 00:00:00pages
1-6eissn
0166-0934issn
1879-0984pii
S0166-0934(14)00368-1journal_volume
210pub_type
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