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Early screening for anti-plum pox virus monoclonal antibodies with different epitope specificities by means of gold-labelled immunosorbent electron microscopy.

Abstract：

:The technique of gold-labelled immunosorbent electron microscopy for the initial screening of monoclonal antibodies 10 days after cell fusion from 96-well culture plates is described. The technique is used to identify clones that secrete antibodies binding on the surface of the virion or to viral subunits, and compared to ELISA and Western blotting. High sensitivity was demonstrated.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Himmler G,Brix U,Steinkellner H,Laimer M,Mattanovich D,Katinger HW

doi

10.1016/0166-0934(88)90118-8

subject

Has Abstract

pub_date

1988-12-01 00:00:00

pages

351-7

issue

2-3

eissn

0166-0934

issn

1879-0984

pii

0166-0934(88)90118-8

journal_volume

22

pub_type

杂志文章

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