Abstract：
:5-min boiling of 3-fold diluted serum leads to the quantitative release of immune-complexed HIV antigen (Ag) that can be readily measured. The method is capable of detecting as little as 4 to 5 pg/ml Ag p24 depending on the Ag test used. Of a panel of 50 anti-HIV-positive sera, 30 (60%) were Ag-positive after heat denaturation, 17 (34%; P < or = 0.01) after acidification, and 13 (26%; P < or = 0.001) when tested undenatured. The superiority of heat denaturation was greatest in sera with high-titered antibodies to p24. Occurrence and mean concentrations of heat-denatured Ag were reversely correlated with p24-specific antibody. It is suggested that heat-denatured proteins be used for raising antibodies that will serve as capture or trace antibodies in Ag assays. This simple change in the manufacturing of test reagents in combination with a short boil before testing may solve the problem of incomplete Ag detection due to immune complex formation.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Schüpbach J,Böni Jdoi
10.1016/0166-0934(93)90080-bsubject
Has Abstractpub_date
1993-07-01 00:00:00pages
247-56issue
2eissn
0166-0934issn
1879-0984pii
0166-0934(93)90080-Bjournal_volume
43pub_type
杂志文章abstract：:This study assesses the ability of quantitative real-time PCR to measure the effects of virus DNA polymerase inhibitors on EBV DNA and late mRNAs syntheses in EBV-producing cell lines. In-house real-time quantitative PCRs were used to measure EBV DNA (thymidine kinase) and mRNAs (BLLF1 gene/gp350/220, BVRF2 gene/prote...
journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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doi：10.1016/0166-0934(91)90106-a
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(87)90034-6
更新日期：1987-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
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更新日期：1980-01-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(83)90026-5
更新日期：1983-03-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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更新日期：2000-03-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2012.02.005
更新日期：2012-05-01 00:00:00
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journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2010.08.013
更新日期：2010-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2005-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2000-01-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2004-10-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2008-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(95)01984-7
更新日期：1996-04-05 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1988-06-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2021-02-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2014.07.028
更新日期：2014-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(85)90121-1
更新日期：1985-05-01 00:00:00
abstract：:In vitro enzymatic amplification was applied to detect hepatitis B virus (HBV) DNA sequences in serum. This technique, known as the polymerase chain reaction (PCR) was used to amplify a 128 bp DNA fragment including a 112 nucleotide long sequence complementary to a region in the S gene of the HBV genome. Amplified sam...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(88)90126-7
更新日期：1988-07-01 00:00:00
