Abstract:
:A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the real-time PCR established in this study enabled detection of as little as 10(2) copies of SLCV DNA with CP gene as the target. This TaqMan real-time PCR assay for detection and quantitation of SLCV would be a useful tool for application in quarantine and certification of SLCV in cucurbits as well as in the research of disease resistance and epidemiology.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Kuan CP,Huang HC,Chang CC,Lu YLdoi
10.1016/j.jviromet.2011.11.024subject
Has Abstractpub_date
2012-02-01 00:00:00pages
367-72issue
2eissn
0166-0934issn
1879-0984pii
S0166-0934(11)00461-7journal_volume
179pub_type
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