Abstract:
:High-titered phage stocks are required for carrying out genetic and physicochemical studies on bacteriophages. This study describes a simple method for the concentration of coliphages MS-2 and T2 by charge-modified filters. Phage lysates were first clarified by filtration through serum-coated membrane filters. The clarified lysate was adjusted to pH 6 and passed through a Zeta-plus filter (30 S size). Greater than 99% virus adsorption occurred under these conditions. Adsorbed viruses were successfully eluted by small volumes of 3% beef extract (pH 10) which was subsequently neutralized with 3% beef extract (pH2). A concentration factor as great as 126 could be achieved by this method, with recoveries ranging from 54% to 97% (average = 60%).
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Goyal SM,Zerda KS,Gerba CPdoi
10.1016/0166-0934(80)90016-6subject
Has Abstractpub_date
1980-01-01 00:00:00pages
79-85issue
2eissn
0166-0934issn
1879-0984pii
0166-0934(80)90016-6journal_volume
1pub_type
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