Abstract：
:The recently developed semi-automatic Hepatube system was evaluated in comparison to another radioimmunoassay for the detection of hepatitis B surface antigen (HBsAg), the manual Ausria II-125 test. After incubation of serum in anti-HBs coated tubes, the Hepatube system uses a machine to wash the tubes and to add tracer. After a second incubation, tubes are washed again in the machine and are manually transferred to the gamma counter. Two machines were used. Machine 1 had an undefined defect. Of 1490 samples tested, 69 (4.6%) gave false-positive results versus 11 (0.7%) in the Ausria II-125 test. Machine 2 had one false-positive result among 920 samples versus 5 in the Ausria II-125 test. The sensitivity was measured with reference panels from Wellcome and Abbott as well as in titration series. The Hepatube system was found to be a factor three less sensitive than the Ausria II-125 test. The Hepatube processor is easy to handle; radioactive material can be held at a distance during the whole procedure; waste material is limited and less voluminous than in the Ausria II-125 test.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
de Vries J,Kruining J,Heijtink RAdoi
10.1016/0166-0934(83)90074-5subject
Has Abstractpub_date
1983-02-01 00:00:00pages
107-15issue
2eissn
0166-0934issn
1879-0984pii
0166-0934(83)90074-5journal_volume
6pub_type
杂志文章abstract：:The humoral immune response of sheep infected with bluetongue virus serotypes 3, 9 and 16 was monitored by plaque inhibition (PI), blocking ELISA (BELISA) and indirect ELISA over a period of 63 days post-infection. Results indicated that testing of a single plasma or serum sample by both a BELISA and an indirect ELISA...
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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更新日期：2002-01-01 00:00:00
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更新日期：1982-08-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1987-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jviromet.2013.02.005
更新日期：2013-05-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1994-04-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：2019-08-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2013.07.058
更新日期：2013-11-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/0166-0934(91)90009-o
更新日期：1991-06-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2007.10.026
更新日期：2008-03-01 00:00:00
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
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journal_title：Journal of virological methods
pub_type： 杂志文章
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更新日期：1984-12-01 00:00:00
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journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2008.02.004
更新日期：2008-05-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2018-03-01 00:00:00
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journal_title：Journal of virological methods
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更新日期：2001-06-01 00:00:00
abstract：:Hepatitis E virus (HEV) is the causative agent of hepatitis E, a food- and water-borne disease. In developed countries, consumption of meats from pigs, wild boars and deer is a major source of infection. Although HEV and HEV-related viruses have been detected in many animal species, their zoonotic potential and preval...
journal_title：Journal of virological methods
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更新日期：2016-12-01 00:00:00
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journal_title：Journal of virological methods
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doi：10.1016/j.jviromet.2009.09.023
更新日期：2010-02-01 00:00:00
abstract：OBJECTIVE:This study evaluated the performance of the cobas® hepatitis C virus (HCV) Test for use on the cobas® 6800/8800 Systems for the detection and quantification of HCV RNA collected using the cobas® Plasma Separation Card (PSC) compared with plasma samples. METHODS:Whole EDTA-venous blood was collected from 50 H...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2020.113820
更新日期：2020-04-01 00:00:00
abstract：:It remains unclear whether hepatitis B virus (HBV) replicates in extrahepatic tissues, and particularly in peripheral blood mononuclear cells (PBMCs), which may serve as a reservoir for the maintenance of infection. A real-time PCR assay for the quantitation of total and covalently closed circular (ccc) HBV DNA in ser...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2006.07.019
更新日期：2006-12-01 00:00:00
abstract：:To formulate inactivated influenza vaccines, the concentration of hemagglutinin (HA) must be accurately determined. The standard test currently used to measure HA in influenza vaccines is the Single Radial Immunodiffusion (SRID) assay. We developed a very rapid, simple and sensitive alternative quantitative HA assay, ...
journal_title：Journal of virological methods
pub_type： 杂志文章
doi：10.1016/j.jviromet.2017.10.011
更新日期：2018-01-01 00:00:00
