Abstract:
:Viral susceptibility testing has been traditionally performed by the plaque reduction assay (PRA) which is laborious, time consuming, relatively expensive, and requires subjective input by the reader. An in situ cellular enzyme-linked immunosorbent assay (ELISA) has been developed with the potential to overcome many of the limitations of PRA and has been applied to a variety of viruses. This study establishes the specific conditions necessary for susceptibility testing of influenza A virus to antiviral agents such as amount of inoculum size, duration of incubation, fixative type, and cell number; factors which are critical to the performance of the in situ cellular ELISA. In situ cellular ELISA was found to correlate strongly with the plaque assay (PA) (R2 = 0.997, P < 0.002). Both assays were applied to test the susceptibility of influenza A virus to a new antiviral emulsion agent and yielded comparable data. The optimized in situ cellular ELISA can serve as a reliable assay for the rapid screening of large numbers of antiviral agents.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Myc A,Anderson MJ,Baker JR Jrdoi
10.1016/s0166-0934(98)00150-5subject
Has Abstractpub_date
1999-02-01 00:00:00pages
165-77issue
2eissn
0166-0934issn
1879-0984pii
S0166093498001505journal_volume
77pub_type
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