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Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein.

Abstract：

:Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10-25 residues. Following analyses, 2 peptides spanning the 436-445 and 437-461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Chang HH,Chen PK,Lin GL,Wang CJ,Liao CH,Hsiao YC,Dong JH,Sun DS

doi

10.1016/j.jviromet.2014.01.022

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

1-6

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(14)00032-9

journal_volume

201

pub_type

杂志文章

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