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Comparison of a conventional HIV 1/2 line immunoassay with a rapid confirmatory HIV 1/2 assay.

Abstract：

:The performance of the rapid confirmatory HIV 1/2 assay Geenius was compared with the conventional HIV 1/2 line immunoblot (INNO-LIA HIV I/II Score). One hundred HIV 1/2 confirmed positive samples from donors and patients and 136 negative screening samples from blood donors were evaluated with both assays. A 20 member performance panel consisting of different HIV 1 and 2 subtypes was also analysed. Ninety-nine of the confirmed HIV positive samples were positive with both assays. One sample was positive with the INNO-LIA HIV I/II Score but indeterminate with the Geenius HIV 1/2. From 136 negative blood donor samples (negative with a combo HIV assay and a highly sensitive ID-NAT), 125 were concordant negative. Six and five samples were incorrectly indeterminate with the INNO-LIA HIV I/II Score and the Geenius HIV 1/2, respectively. One sample was weak positive with the INNO-LIA HIV I/II Score but negative with the Geenius HIV 1/2. The 20 member performance showed equivalent results with both assays. The rapid assay showed a comparable sensitivity and specificity for confirmation for positive and negative HIV donor and patient samples as well for a 20 member performance panel.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Tinguely C,Schild-Spycher T,Bahador Z,Gowland P,Stolz M,Niederhauser C

doi

10.1016/j.jviromet.2014.05.010

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

1-4

eissn

0166-0934

issn

1879-0984

pii

S0166-0934(14)00200-6

journal_volume

206

pub_type

杂志文章

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