Abstract：
:The diagnostic system based on reverse transcription (RT)-PCR has been used widely for the detection of viral genomes of faecal-borne RNA viruses. However, faecal specimens often produce both false positive and false negative results. Therefore, there is a need for a diagnosis procedure that can control for 'false-results'. In this study, an internal standard RNA that can serve as a non-competitive positive template was developed and used directly to detect faecal-borne RNA viruses without noticeable competitive inhibition of the target viral genome. These results suggest that the internal standard RNA is a useful standard molecule when undertaking diagnostic qualitative RT-PCR procedures for enteroviruses and related faecal-borne RNA viruses.
journal_name
J Virol Methodsjournal_title
Journal of virological methodsauthors
Kim K,Park J,Chung Y,Cheon D,Lee IB,Lee S,Yoon J,Cho H,Song C,Lee KHdoi
10.1016/s0166-0934(02)00016-2subject
Has Abstractpub_date
2002-07-01 00:00:00pages
107-15issue
2eissn
0166-0934issn
1879-0984pii
S0166093402000162journal_volume
104pub_type
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