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Effects of codon-optimization on protein expression by the human herpesvirus 6 and 7 U51 open reading frame.

Abstract：

:Codon-optimization refers to the alteration of gene sequences, to make codon usage match the available tRNA pool within the cell/species of interest. Codon-optimization has emerged as a powerful tool to increase protein expression by genes from small RNA and DNA viruses, which commonly contain overlapping reading frames as well as structural elements that are embedded within coding regions; these features are not widespread among large DNA viruses. We therefore examined whether codon-optimization might influence protein expression from a herpesvirus gene. We focused on the U51 gene from human herpesviruses-6 and -7, which was cloned in both native and codon-optimized form, with an N-terminal HA epitope tag to allow protein detection. Codon-optimization was associated with a profound (10-100 fold) increase in U51 expression in human (293A, HSG, K562) or hamster (CHO) cell lines, suggesting this may represent a valuable tool to facilitate functional studies on recalcitrant herpesvirus genes. Finally, it is postulated that the suboptimal expression of native U51 may reflect a regulatory mechanism that controls viral gene expression.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Bradel-Tretheway BG,Zhen Z,Dewhurst S

doi

10.1016/s0166-0934(03)00173-3

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

145-56

issue

2

eissn

0166-0934

issn

1879-0984

pii

S0166093403001733

journal_volume

111

pub_type

杂志文章

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