当前位置： SCI文献检索 > JOURNAL OF VIROLOGICAL METHODS期刊下所有文献 > Determination of tumor necrosis factor-alpha levels in dengue virus infected patients by sensitive biotin-streptavidin enzyme-linked immunosorbent assay.

Determination of tumor necrosis factor-alpha levels in dengue virus infected patients by sensitive biotin-streptavidin enzyme-linked immunosorbent assay.

Abstract：

:A modified sandwich enzyme-linked immunosorbent assay using biotin-streptavidin system (BS-ELISA) was developed to determine levels of tumor necrosis factor-alpha (TNF-alpha) in serum samples of children infected with dengue virus (n=99) and healthy controls (n=41). The minimum detectable concentration of TNF-alpha by the BS-ELISA was 3.3 pg/ml. The mean TNF-alpha level was highest in those patients with dengue shock syndrome (DSS) or dengue hemorrhagic fever (DHF) grade III (37.44+/-42.0 pg/ml). Lower levels were found in DHF grade I (28.44+/-42.7 pg/ml), DHF grade II (24. 21+/-25.4 pg/ml) and dengue fever (DF) (14.10+/-24.0 pg/ml). TNF-alpha in the sera of DF and DHF patients could be detected on days 2-6 after the onset of fever, the high level occurring on day 5. TNF-alpha was detected in 41.4% (24.01+/-35.2 pg/ml) of dengue virus infected patients and 7.3% (4.2+/-15.6 pg/ml) of control subjects. The sera of patients contained significantly higher levels of TNF-alpha than the sera of controls, P-value<0.001. DHF patients had significantly higher levels of TNF-alpha than DF patients (P-value=0.020) but no difference in the TNF-alpha levels from sera of DHF grades I-III patients was observed (P-value=0.295). The results indicate that the BS-ELISA is a very sensitive method for determining TNF-alpha in serum samples of DF and DHF patients. The TNF-alpha levels might be associated with dengue virus infection and related to disease severity of DHF.

journal_name

J Virol Methods

journal_title

Journal of virological methods

authors

Kittigul L,Temprom W,Sujirarat D,Kittigul C

doi

10.1016/s0166-0934(00)00215-9

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

51-7

issue

1

eissn

0166-0934

issn

1879-0984

pii

S0166093400002159

journal_volume

90

pub_type

杂志文章

相关文献

JOURNAL OF VIROLOGICAL METHODS文献大全
  • Optimisation and sensitivity of single-stranded conformation polymorphism for the detection of hepatitis C virus quasi-species.

    abstract：:Single-stranded conformation polymorphism (SSCP) is a technique used widely for the detection of differences in DNA sequence based on PCR technology. Developed by geneticists for the detection of mutations causing disease, it has been adopted more recently for the analysis of the quasi-species of viral genomes, such a...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(00)00279-2

    authors： McKechnie VM,McCruden EA

    更新日期：2001-04-01 00:00:00

  • Real-time PCR assay to identify variants of Vaccinia virus: implications for the diagnosis of bovine vaccinia in Brazil.

    abstract：:Naturally occurring infections of Vaccinia virus (VACV) have been recognized in Brazil during the past 10 years. Human Brazilian Vaccinia virus (BVV) infections typically occur as a zoonosis transferred from affected dairy cows to their handlers. Outbreaks have caused notable economic losses to the rural community in ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2008.05.028

    authors： de Souza Trindade G,Li Y,Olson VA,Emerson G,Regnery RL,da Fonseca FG,Kroon EG,Damon I

    更新日期：2008-09-01 00:00:00

  • Sensitive analytic ELISAs for subclass herpes virus IgG.

    abstract：:The subclass distribution of antiviral antibodies to three herpes viruses was studied in a population of healthy blood donors. Subclassification by monoclonal antibodies led to the identification of certain viral IgG patterns. IgG1 appeared to be formed in response to almost all CMV, HSV-1 and VZV infections. A higher...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(85)90061-8

    authors： Gilljam G,Sundqvist VA,Linde A,Pihlstedt P,Eklund AE,Wahren B

    更新日期：1985-03-01 00:00:00

  • Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus.

    abstract：:Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assa...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.11.003

    authors： Binkhamis K,Gillis H,Lafreniere JD,Hiebert J,Mendoza L,Pettipas J,Severini A,Hatchette TF,LeBlanc JJ

    更新日期：2017-01-01 00:00:00

  • Detection of human papillomavirus DNA and mRNA using synthetic, type-specific oligonucleotide probes.

    abstract：:Type-specific 30'mer-36'mer oligonucleotide probes complementary to mRNA transcribed from the E6 and E7 open reading frames of HPV 6b/11, 16, 18 and 33 were designed using the published nucleotide sequences. As oligonucleotides are easily and relatively cheaply synthesized in large amounts and are free of vector DNA, ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(93)90018-m

    authors： Faulkner-Jones BE,Tabrizi SN,Borg AJ,Roche PJ,Haralambidis J,Coghlan JP,Garland SM

    更新日期：1993-03-01 00:00:00

  • Rescue of measles virus using a replication-deficient vaccinia-T7 vector.

    abstract：:A system which allows the reconstitution of measles virus (MV) from cloned cDNA is described. The severely host cell restricted vaccinia vector MVA-T7 expressing bacteriophage T7 RNA polymerase was used to generate full-length antigenomic MV RNA and simultaneously the mRNAs encoding the viral N, P and L proteins in or...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(96)02137-4

    authors： Schneider H,Spielhofer P,Kaelin K,Dötsch C,Radecke F,Sutter G,Billeter MA

    更新日期：1997-02-01 00:00:00

  • Treatment of persistent active herpesvirus infections.

    abstract：:All human herpesviruses cause chronic infections in which latent virus is periodically reactivated. Persistently active infections are uncommon, however, and occur exclusively in individuals whose immune systems fail to control virus multiplication and spread. This paper summarizes the management of these unusual infe...

    journal_title：Journal of virological methods

    pub_type： 杂志文章,评审

    doi：10.1016/0166-0934(88)90076-6

    authors： Straus SE

    更新日期：1988-09-01 00:00:00

  • Colonisation of mice and pigs by a chimeric porcine circovirus 1-2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines.

    abstract：:A chimeric porcine circovirus (PCV) 1-2b vaccine strain and its parental wild-type PCV2b strain from China (PCV2-J) were used separately to vaccinate BALB/c mice and tissue and serum samples were collected from the mice to investigate whether the replication properties of the viruses differed. The spleen lymphocytes f...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2020.113905

    authors： Cui X,Wang X,Gao Q,Liu X,Kai Y,Chen C,Gao S

    更新日期：2020-09-01 00:00:00

  • The development of a real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay using TaqMan technology for the pan detection of bluetongue virus (BTV).

    abstract：:Bluetongue virus (BTV) is an infectious, non-contagious viral disease of domestic and wild ruminants that is transmitted by adult females of certain Culicoides species. Since 2006, several serotypes including BTV-1, 2, 4, 6, 8, 9 and 16, have spread from the Mediterranean basin into Northern Europe for the first time....

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2017.03.009

    authors： Mulholland C,McMenamy MJ,Hoffmann B,Earley B,Markey B,Cassidy J,Allan G,Welsh MD,McKillen J

    更新日期：2017-07-01 00:00:00

  • Improvement of a quantitative cell tropism assay for rapid and reliable characterization of human cytomegalovirus mutants.

    abstract：:Endothelial cell tropism is an inherent property of isolates of human cytomegalovirus (HCMV), and has been proposed as a pathogenicity factor of HCMV. Mutational approaches can be applied for analysis of the molecular determinants of endothelial cell tropism, but the evaluation of mutants is limited by the low reliabi...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2010.03.031

    authors： Schuessler A,Sampaio KL,Sinzger C

    更新日期：2010-08-01 00:00:00

  • An immunochromatography assay for rapid antemortem diagnosis of dogs suspected to have canine distemper.

    abstract：:A new assay was developed for rapid and antemortem diagnosis of canine distemper (CD). This immunochromatography (IC)-based assay, which employs two monoclonal anti-CDV antibodies, was compared with nested PCR. When serial dilutions of purified CDV were tested, the CDV detection limits of the nested PCR and IC assays ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.09.006

    authors： An DJ,Kim TY,Song DS,Kang BK,Park BK

    更新日期：2008-02-01 00:00:00

  • Monoclonal antibody against Saint Louis encephalitis prM viral protein.

    abstract：:Saint Louis encephalitis virus belongs to Flavivirus genus; Flaviviridae family jointly with other medically important flaviviruses including dengue virus and West Nile virus. The biological properties and functions of prM flavivirus protein are under investigation due to its importance in the generation of infectious...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.01.004

    authors： Pupo-Antúnez M,Vázquez S,Sosa AL,Caballero Y,Vásquez Y,Morier L,Álvarez M,Guzmán MG

    更新日期：2015-06-15 00:00:00

  • Evaluation of currently available bovine viral diarrhoea virus (BVDV) and HoBi-like pestivirus (HoBiPeV) specific diagnostic tests in detection of highly divergent HoBiPeVs in cattle.

    abstract：:The emergence of novel and divergent HoBi-like pestivirus (HoBiPeV) strains in cattle in Asia recently has raised concerns with regard to their reliable and accurate diagnosis. Hence, the aim of this study was to evaluate currently available BVDV diagnostic tests and HoBiPeV-specific diagnostic tests in detection of g...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2019.113707

    authors： Moorthy D,Mishra N,Kalaiyarasu S,Jhade SK,Singh VP

    更新日期：2019-10-01 00:00:00

  • Comparison of an antibody-capture IgM enzyme-linked immunosorbent assay with IgM-indirect immunofluorescence for the diagnosis of acute Sindbis and West Nile infections.

    abstract：:The development and evaluation of an antibody-capture ELISA for the detection of IgM antibodies to Sindbis (SIN) and West Nile (WN) viruses are described. Comparison of the ELISA results with those obtained by indirect immunofluorescence (IIF) antibody tests using both fluorescein and biotinylated anti-human IgM conju...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(89)90060-8

    authors： Besselaar TG,Blackburn NK,Aldridge N

    更新日期：1989-09-01 00:00:00

  • High yield expression and purification of Chikungunya virus E2 recombinant protein and its evaluation for serodiagnosis.

    abstract：:Disease caused by Chikungunya virus (CHIKV) is clinically characterized by sudden-onset of fever and severe arthralgia, which may persist for weeks, months, or years after acute phase of the infection. CHIKV is spreading globally; in India it first appeared in the 1960s followed by a quiescent period and then a full-b...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2016.05.003

    authors： Verma A,Chandele A,Nayak K,Kaja MK,Arulandu A,Lodha R,Ray P

    更新日期：2016-09-01 00:00:00

  • Generation of a porcine alveolar macrophage cell line for the growth of porcine reproductive and respiratory syndrome virus.

    abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) has a marked tropism for cells of the monocyte-macrophage lineage and accordingly, replicates in fully differentiated alveolar macrophages in the natural host. Despite the identification of several putative receptors for PRRSV on porcine alveolar macrophages ...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.11.003

    authors： Lee YJ,Park CK,Nam E,Kim SH,Lee OS,Lee du S,Lee C

    更新日期：2010-02-01 00:00:00

  • Comparison of two commercial kits and an in-house ELISA for the detection of equine rotavirus in foal feces.

    abstract：:Group A rotaviruses (RVA) are important infectious agents associated with diarrhea in the young of several animal species including foals. Currently, a variety of diagnosis methods are commercially available, like ELISA, latex agglutination and immunochromatographic assays. These commercial tests are mainly designed f...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2015.05.002

    authors： Miño S,Kern A,Barrandeguy M,Parreño V

    更新日期：2015-09-15 00:00:00

  • Epitope mapping of the NS4 and NS5 gene products of hepatitis C virus and the use of a chimeric NS4-NS5 synthetic peptide for serodiagnosis.

    abstract：:Specific domains of the NS4 and NS5 gene products of hepatitis C virus have been identified using hydrophilicity profiles for the prediction of potential immunogenic regions, and epitope scanning techniques. Peptides synthesised on the basis of such data show excellent reactivity in the ELISA format. Introduction of a...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(95)00060-8

    authors： Rosa C,Osborne S,Garetto F,Griva S,Rivella A,Calabresi G,Guaschino R,Bonelli F

    更新日期：1995-10-01 00:00:00

  • Generation of dried tube specimen for HIV-1 viral load proficiency test panels: a cost-effective alternative for external quality assessment programs.

    abstract：:Participation in external quality assessment programs is critical to ensure quality clinical laboratory testing. Commercially available proficiency test panels for HIV-1 virus load testing that are used commonly in external quality assessment programs remain a financial obstacle to resource-limited countries. Maintain...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2012.11.036

    authors： Ramos A,Nguyen S,Garcia A,Subbarao S,Nkengasong JN,Ellenberger D

    更新日期：2013-03-01 00:00:00

  • Interferon and antibody titrations using haemagglutinating Togaviridae and trypsinized human erythrocytes.

    abstract：:Several Togaviridae of the alphavirus and flavivirus genera agglutinate trypsinized human group O erythrocytes (THOE) (Shortridge and Hu, 1976). Haemagglutinin titers of Semliki Forest virus (SFV) and Japanese encephalitis virus (JEV) measured with THOE were equivalent to, if not higher than, those obtained with Embde...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(83)90073-3

    authors： Sedmak JJ,Dixon M,Schoenherr C,Sabran JL,Grossberg SE

    更新日期：1983-02-01 00:00:00

  • Clinical utility of 2',5'-oligoadenylate synthetase activity measurement: using whole blood as a highly sensitive method to detect the effects of IFN.

    abstract：:Research progress on the pleiotropic effects of interferons (IFN) has thus far required detecting responses by weak IFN signals. The activity of 2',5'-oligoadenylate synthetase (2-5OAS) is a valuable indicator in the prognosis and IFN treatment of patients with viral diseases such as hepatitis B and C. Although serum ...

    journal_title：Journal of virological methods

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.jviromet.2006.05.005

    authors： Uno K,Suginoshita Y,Kakimi K,Moriyasu Y,Nakano K,Nakamura N,Fujita T,Horino Y,Sato T,Kishida T

    更新日期：2006-09-01 00:00:00

  • Simultaneous detection of enterovirus 70 and coxsackievirus A24 variant by multiplex real-time RT-PCR using an internal control.

    abstract：:Epidemics of acute hemorrhagic conjunctivitis are always explosive and extensive, and have been recognized as a serious international public health problem. Enterovirus 70 and coxsackievirus A24 variant have been identified as the major etiological agents in acute hemorrhagic conjunctivitis outbreaks worldwide. A nove...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2009.02.022

    authors： Xiao XL,Wu H,Li YJ,Li HF,He YQ,Chen G,Zhang JW,Yang H,Li XF,Yang XQ,Yu YG

    更新日期：2009-07-01 00:00:00

  • In vitro replication of Macrobrachium rosenbergii nodavirus and extra small virus in C6/36 mosquito cell line.

    abstract：:White tail disease (WTD) is a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India and many parts of the world. The pathogenic agents have been identified as M. rosenbergii nodavirus (MrNV) associated with extra small virus (XSV), which is 27nm and 15nm in diameter, respectively. Repli...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2007.06.011

    authors： Sudhakaran R,Parameswaran V,Sahul Hameed AS

    更新日期：2007-12-01 00:00:00

  • A novel intergenic site for integration and expression of foreign genes in the genome of pseudorabies virus.

    abstract：:Restriction enzyme analysis of DNA of a number of Pseudorabies virus (PRV) single plaque isolates revealed in several cases the existence of a unique EcoRI cleavage site, which has not been observed in PRV DNA before. This EcoRI site was mapped to the right end of the unique long region of the PRV genome, in BamHI-fra...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(94)90019-1

    authors： Fuchs W,Bauer B,Mettenleiter TC,Rziha HJ

    更新日期：1994-01-01 00:00:00

  • Predicting herd protection against foot-and-mouth disease by testing individual and bulk tank milk samples.

    abstract：:Four groups of cattle were tested for antibodies against foot-and-mouth disease (FMD) virus type O(1) over three 70 day vaccination cycles using the liquid-phase-blocking-ELISA (LPBE). First lactation cows showed the lowest titres and group protection levels (GPLs) against FMD virus strains with 'r' values < or =0.5 w...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(01)00342-1

    authors： Armstrong RM,Mathew ES

    更新日期：2001-09-01 00:00:00

  • Immunoblots may not be effective in confirming the recency of HIV-1 infection.

    abstract：:Recently, immunoblots (IBs) have tended to substitute Western blots (WBs) for HIV infection diagnosis. Several studies have confirmed IBs' high sensitivity to confirm HIV infection for every stage. Since the nature and pattern of the antigens of IBs are different from those of WB, the abilities of IBs and WBs to disti...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/j.jviromet.2021.114074

    authors： Mariaggi AA,Gardiennet E,Stefic K,Essat A,Cheret A,Goujard C,Meyer L,Barin F,Avettand-Fenoel V,ANRS PRIMO cohort.

    更新日期：2021-01-21 00:00:00

  • A quantitative duplex PCR technique for measuring amounts of cell-associated Marek's disease virus: differences in two populations of lymphoma cells.

    abstract：:A duplex polymerase chain reaction (PCR) was developed to measure Marek's disease virus (MDV) load in two subpopulations of Marek's disease (MD) lymphoma cells from chickens. PCR primers were designed using the sequence of the MDV-ICP4 gene and the chicken IFNgamma gene. Each set of primers was present in the same rea...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/s0166-0934(99)00075-0

    authors： Burgess SC,Davison TF

    更新日期：1999-09-01 00:00:00

  • Poly A-linked non-isotopic microtiter plate reverse transcriptase assay for sensitive detection of clinical human immunodeficiency virus isolates.

    abstract：:A colorimetric reverse transcriptase assay (cRT assay) was developed for quantitative detection of HIV-1. In this format, reverse transcriptase incorporates biotin-labeled dUTP onto oligo-dT primers hybridized to poly A templates. The templates are covalently bound to the surface of microtiter wells. The amount of inc...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(95)00073-5

    authors： Suzuki K,Saito T,Kondo M,Osanai M,Watanabe S,Kano T,Kano K,Imai M

    更新日期：1995-11-01 00:00:00

  • Rapid biotin-avidin method for quantitation of antiviral antibody isotypes.

    abstract：:A rapid and efficient method is described for isotype quantitation of antiviral antibodies in mice infected with Theiler's murine encephalomyelitis virus (TMEV). Serum antibodies were reacted with fluorochrome-labeled TMEV in a modified fluid-phase particle concentration fluorescence immunoassay (PCFIA). Biotin and av...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(90)90135-3

    authors： Peterson JD,Miller SD,Waltenbaugh C

    更新日期：1990-02-01 00:00:00

  • Epitope-mapping on the Epstein-Barr virus major capsid protein using systematic synthesis of overlapping oligopeptides.

    abstract：:Systematic solid-phase synthesis of all possible overlapping nonapeptides of the 1381 amino acid sequence of the Epstein-Barr virus major capsid protein (EBV-MCP) was used to identify the position of linear antigen epitopes on this protein as recognised by human polyclonal antisera. Antisera were selected for reactivi...

    journal_title：Journal of virological methods

    pub_type： 杂志文章

    doi：10.1016/0166-0934(88)90061-4

    authors： Middeldorp JM,Meloen RH

    更新日期：1988-09-01 00:00:00